Why crystal like structures are formed on NGM plates cultured with E. coli OP50?

When I prepare NGM plate for the growth of C. elegans, the plates are clear. However, when I spread culture of E. coli OP50 on NGM plates and incubate at 37 C overnight, I observed crystal like...

10 November 2017 707 4 View

Any advice on the difference in the Ct value of reference gene in treated and control samples?

I am using 16S primers for qPCR. However I got different Ct values in may treated and control sample (30.08 and 28.85). Would it lead to considerable error in final results? In my opinion,...

06 July 2015 3,326 10 View

Is it necessary to dilute cDNA before qPCR? How much dilution should be made? How to determine best dilution factor?

Kindly explane importance of cDNA dilution before qPCR and how one can determine best dilution factor for cDNA samples? How it will effect results of qPCR? In other words, how much quantity of...

05 June 2015 1,350 22 View

Is it possibile to determine genomic DNA contamination in cDNA sample on the basis of qPCR results?

Is there any possible way to find out genomic DNA contamination/residues in cDNA by analyzing results of qPCR? Can we get any information related to genomic DNA by Ct value?

05 June 2015 738 4 View

Which are experimentally proved Best house keeping genes to study gene expression in Pseudomonas syringae?

I need some information about house keeping genes to be used as internal control particularly for Pseudomonas syringae. Kindly let me know which house keeping genes are good to study gene...

31 December 2014 5,607 4 View

How can I avoid contamination of Genomic DNA during RNA extraction?

It is observed that RNA samples are often contaminated with genomic DNA. How can this contamination be avoided? What are the possible causes for genomic DNA contamination during mRNA extraction.

31 December 2014 5,040 3 View

We want to remove the gaps/errors of our laboratory genomes with some reference genomes. Which software would be the best option?

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09 October 2014 9,468 15 View

How can one convert genome sequence files of microsoft office into genbank format?

I have a genome sequence however, sequence is in microsoft word file and annotation is present in excel file. How can I convert/merge this information into genbank format. (sequence is not...

09 October 2014 6,644 6 View

What software/database could be used for identification of pan genome/core genome of user's own sequence data?

1) The sequence data is in contigs form. 2) Please suggest windows based tools. 3) Important literature/source would also be appreciated.

08 September 2014 8,349 18 View

What techniques are best to confirm construction of complementary strain?

After constructing a mutant bacterial strain, I have to construct complementary strain. How can I confirm expression of gene of interest in complementary strain? Which techniques can be used for...

05 June 2014 9,967 1 View

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02 March 2021 3,060 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View