You can quantify PGE2 production by macrophages cell lines using a immune enzimatic assay kit. Reduction of PGE2 synthesis could be related with inhibition of COX activity.
take a look in this paper
In vitro and in vivo anti-inflammatory activities of columbin through the inhibition of
cycloxygenase-2 and nitric oxide but not the suppression of NF-κB translocation
This could also be a way of assaying cox inhibition. The COX Inhibitor Screening Assay directly measures PGF2α produced by SnCl2 reduction of COX-derived PGH2. The prostanoid product is quantified via enzyme immunoassay (EIA) using a broadly specific antibody that binds to all the major prostaglandin compounds.
Hello,
Is it possible to assessed the capacity of phytochemicals inhibiting COX-2 through an enzymatic assay? I'm know Cayman has a colorimetric method, although very expensive once I have a wide range of samples and concentrations to test. Do you know and can provide a good enzymatic protocol?
Thanks in advance!
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