Carolina Pereira

4 Questions 9 Answers 0 Followers

Questions related from Carolina Pereira

What is the best stimulation to detect IL-10 and IL-6 on Caco-2 by ELISA?

Does anyone know which stimulus can i use to detect Il-10 and IL-6 on Caco-2? I have cultured Caco-2 for 21 days in a 12-well plate and did the stimulation with (25 ng/mL IL1b, 10 ug/mL LPS, 50...

09 September 2018 4,875 2 View

Does anyone know how I can know which anion has the most hydrophilic character?

I'm working with choline-based ionic liquids where the anions differ from: acetate, hexanoate, isobutyrate and propionate. I analyzed the contact angle using water droplet and obtained increased...

09 September 2016 7,338 6 View

How can I overcome solubility problems with Polymethoxyflavones?

I'm testing polymethoxyflavones in cell assays (Nobiletin/Tangeretin/Sinensetin) and in higher concentration tested, these PMF's showed crystallization. The stock solution are diluted in DMSO. Can...

02 February 2016 3,943 1 View

How do I avoid crystallized compounds during cell assays with longer incubation times?

I'm doing antiproliferative assays with different incubation times. I had the compound (previously diluted in DMSO) together with cell culture medium for 24h, 48h or 72h doing nothing during that...

11 November 2015 5,429 4 View