Is school related gender based violence under the field of inclusive education?

The social concern of school related gender based violence has been trending lately in various education research journals and discussions around psycho-social well being of learners. I need...

11 May 2020 3,131 7 View

How to solve temperature issue of molecular dynamics in vasp?

Both beginning temp and target temp are 300 K. Why doesn't this reach 300 K? Please refer to picture that I attached INCAR file goes like this ENCUT = 400 NSW = 50000 IBRION = 0 ISIF = 2 LREAL...

06 April 2020 7,019 1 View

Is there any atmosphere that Titanium chloride can make fume?

I use TiCl4 to check if there's water in the glovebox. when I opened TiCl4 there were a lot of fumes, but I never guess where the water comes. Because there was no oxygen inside. So I didn't sure...

13 January 2020 9,127 1 View

Is there a perfect purification method for semiconductor precursor?

I tried synthesize Ethyl derivated cyclopentadienyl tris(dimethylamido)Zirconium But I have some problem in purification process I want to remove all the solvent from product (toluene) but it...

10 June 2019 4,889 2 View

How you protect the samples/plates from light?

Hello, Sometimes, I have to protect the samples/plates from light such as ELISA, immunofluorescence, etc. I'm usually perform the procedures under the normal room-light, and cover the...

06 July 2018 7,164 5 View

How can I check the target promoter of my primers?

Hi all, I have primers for ChiP analysis in my lab. Before I start the experiments, I'd like to check this is really target for p16INK4A gene (CDKN2A). Q1. How can I check the target promoter...

04 May 2018 4,371 7 View

What kind of technology is suitable for the surface weight measurement of an opaque thin film ?

What kind of technology is suitable for the surface weight measurement of an opaque thin film ? (the weight is in the range of nanogram)

04 May 2016 9,188 13 View

What is the reason for lines in separating gel?

Hi all ! I've used 30:1 acrylamide/bisacrylamide solution (Bio-Rad) for making WB gel. Once I run the gel, the line in separating gel suddenly showed up (please see the attached figure). It really...

10 January 2016 8,495 8 View

What is the reason for lines in separating gel?

Hi all ! I've used 30:1 acrylamide/bisacrylamide solution (Bio-Rad) for making WB gel. Once I run the gel, the line in separating gel suddenly showed up (please see the attached figure). It really...

31 December 2015 6,094 9 View

Any suggestion on why different predicted band sizes appear for the same protein?

03 April 2015 8,807 6 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

What are some important techniques for protein induction and SDS-PAGE analysis?

Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...

03 March 2021 8,237 3 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View