I just can see beta band of collagen(Type I) in mixed gel.
I need to get 3 band (beta, alpha 1 and alpha 2) of collagen.
I used acetic acid(0.2M) solution to dissolve the collagen.
To create a cell line overexpressing a specific microRNA, I'm planning to synthesize and clone the shRNA sequence into a vector. Would it be sufficient to add only the termination sequence (TTTTT)...
27 May 2024 4,206 0 View
Hello everyone, I am trying to clone the gene of interest for the mRNA hybridization ( in situ) after cloning, and miniprepre and restriction digestion, . I don't get the desired product on the...
04 March 2024 9,719 4 View
In many papers that uses proteoliposome uptake assay of proton-coupled transporters (proton symporters), people compare the results of "proton-dependent liposome assay using different pH in...
07 December 2023 2,671 0 View
Hi, When making liposomes, the lipids in organic solvent are first put in a round-bottom flask and are 'rotated' while getting dried under nitrogen gas to obtain "a uniform thin film" of lipids...
10 November 2023 9,894 1 View
Hi, Many membrane protein Cryo-EM structures are in detergent micelle. I was wondering how you would prove that the membrane transporters purified in detergent are still active? because the...
24 May 2023 8,074 2 View
Hi, I'm trying to learn how to do liposome uptake assays but i can't get understand why some papers do "counter-flow" liposome uptake assays while most of the papers don't. By liposome uptake...
15 May 2023 3,851 1 View
I want to study the relationship between parameters for physical activity in a lifespan and the outcome of pain (binary). I have a longitudinal data with four measurement, hence repeated measures....
16 March 2023 5,923 3 View
Hello every I'm looking for datasets looking at methane emissions in wet soils, be it low-lying soils, wetlands, forest swamps, etc.. Any help would be appreciated. Thanks Jonas
09 March 2023 8,102 4 View
Hi, This is probably a very naive question, but I see that a lot of Cryo-EM structure uses FAB to stabilize the conformation or to increase the size of the protein to solve its structure. How can...
14 February 2023 8,945 1 View
I'm doing western blot, using some drug, All condition and process are same. But only "V" drug treatment groups are show different result in western blot band include beta actin. 1. While...
12 December 2022 8,806 1 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
Palmitic acid presence in aqueous fraction
05 August 2024 8,624 4 View
I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...
05 August 2024 9,805 3 View
I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?
02 August 2024 7,186 6 View
Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...
01 August 2024 4,606 7 View
I've seen articles that primarily focus on alpha and beta activity in the frontal regions, but these studies often compare healthy subjects with those having various pathologies. I haven't seen a...
31 July 2024 7,259 1 View
Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...
30 July 2024 2,867 0 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
Dear Researchers, Is it feasible to distinguish between alpha and alpha prime martensite in the Ti6Al4V alloy under rapid solidification in the as-cast state using XRD, in addition to...
30 July 2024 3,849 0 View