Dear Karine,
Can you please rephrase your question!! Its not clear what you want to know. With the advancement of second generation sequencing fishing out hundreds and thousands of SSR has become an easy jobs, with that sequence you can design the primers and then perform SSR for your desired samples.
Dear Nirjhar,
Thank you for interest in this question.
I am conducting a review study on SSR in plants. And in several studies the calculation of the expected number of SSR in plastid genomes is carried out. This calculation is performed to assess whether the microsatellites have been overrepredented or underrepresented. Then the expected value is divided by the observed value. But I can not understand exactly how the expected number calculation is performed. Many studies use the equation given by De Wachter (1981):
Exp(Mt) = f (M)t (1 − f (M)) [G′(1 − f (M))] + 2L,
G′ = G − tL − 2L + 1
Can this calculation be done with the help of software or in a simplified way? But how?
- Badges
- Science method