How can I calculate the detection limit for an impedimetric biosensor?

29 May 2020 1 4K Report

I'm developing an impedimetric biosensor for lipase.

Dear Karine.

There is no consensus in the detection limit determination in biosensors or other analytical measurements. but I'm currently using "3 sigma" method:

Slod= Sblank + 3σblank

where "σblank" is standar deviation of 10 blank curves, "Sblank" is signal of blank curves. You can also calculate LoD by the equation below

LoD= 3*σblank/ (slope of linear part of calibration curves).

Best Regards

Badges
Science topic

More Karine Santos Caetano's questions See All

How we explore a good research finding in this group?

I am a new researcher in Public Health just joined this group and set networking with you all

17 December 2023 9,657 1 View

How to make a vertical and double axis graph on graph Prism?

Here in PJ is what I would like to obtain Thanks to anyone who would have an idea!

01 June 2022 6,107 0 View

Anyone used a Carboxen PLOT 1010 column?

Hello Has anyone used a Carboxen PLOT 1010 column to separate gases such as carbon monoxide, methane and carbon dioxide on GC FID. If so what is the lowest concentration you have managed to get...

19 February 2022 4,073 0 View

At what final concentrations should we use inhibitors of proteases for serum samples, to measure peptides and hormones?

Hello all, To measure the presence of some peptides and hormones in the blood it is important to add, at time of collection to the serum/plasma samples, inhibitors of proteases. At what final...

25 June 2020 6,746 4 View

Figure of gene expression (IL-2/CD40L...) kinetics after T cell activation ?

Dear all, I am looking for the origin of the joined picture that I found on a slide server about the kinetics of gene expression (IL-2/IL-2R/CD40L) followed by proliferation after T cell...

02 January 2020 6,146 2 View

Anyone know where I can get bird's distributional data of oceanic islands?

I need to get data of bird's distribution at marine archipelagoes, but the data need have been islands specific.

05 September 2019 2,860 0 View

Anyone know how to concatenate several protein sequences for phylogenetic analysis?

16 July 2019 5,698 2 View

How calculate summary statistics for approximate Bayesian computation ?

Hi, Currently, I am working on bacterial DNA sequences. I want to calculate summary statistics with mscalc on observed data and simulated data. But how can i convert fasta in ms format? And if i...

14 October 2018 7,503 5 View

Does anyone know if diatom-diazotroph associations are found in freshwater (lakes, rivers) ?

Many different species of oceanic diatoms have been described bearing N2-fixing symbiotic cyanobacteria, I was wondering if similar associations had ever been found in freshwater systems ? Thanks

03 October 2018 1,543 2 View

How can calculation the number of expected SSR ?

23 July 2018 9,712 2 View

Absorption coefficient of methane?

Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?

06 August 2024 980 5 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

How to determine method detection limit in an analytical method?

I know the difference between instrumental LOD and method LOD but my query is - in case of any sample whose concentration is zero or not detected by the instrumental LOD, is it possible to get...

24 July 2024 6,592 5 View

How to determine LOD values?

I am performing fluorescence experiments using a ligand to detect metal ions. I want to determine the Lowest Detection Limit (LOD) using the formula LOD = 3σ / K. However, I'm uncertain about...

19 July 2024 1,086 1 View

Can any of you suggest how to find the detection limit.?

I am working on flourescence sensing of heavy metals using quantum dots.Can any of you suggest how to find the detection limit. Whether it is calculated from formula or we can get from instrument?

19 July 2024 7,411 3 View

Which is the best approach for anomaly detection in scanned image data set?

Anomaly detection in scanned image data set

18 July 2024 3,578 3 View

How to label synapses in over-fixed mice brain sections (40 um) via immunohistochemistry (IHC)?

We have mice brains that were over-fixed due to old PFA used during perfusion. Thus, the synapses are no longer being labeled by the Synaptophysin (SY38) mAB. which works perfectly every other...

17 July 2024 7,767 3 View

How do you decide an optimal AC amplitude for non-faradaic electrochemical impedance spectroscopy?

I work with planar interdigitated electrodes (IDEs). The finger width and the spacing between the fingers are 5 micron. The surface of the IDEs is coated by a non-conductive polymer layer...

16 July 2024 5,031 1 View

Why flow rate of MFC for pure NH3 gas decreases with time?

We have been using MFC to control the flow rate of pure ammonia gas. The MFC is placed in an oven at 50 deg. C to avoid blockage due to NH3. However, after a few days of usage, the MFC is unable...

11 July 2024 5,796 1 View

What are the criteria that must be retained for the development and validation of a qualitative NMR method ?

Dear Community, I would like to develop and validate a qualitative NMR method for the analysis of a specific category of chemicals, and my question is the following: What are the criteria that I...

08 July 2024 1,402 4 View