High GAPDH values in qPCR

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Using two-tailed test for directional hypothesis?

In my study, I have used Process Macro to test the proposed relationships. The hypothesis is directional and two-tailed test has been deployed. Can anyone please clarify how to justify using...

06 February 2021 3,023 3 View

Why a colorimetric assay is usually not in green?

Various colorimetric assays we use are blue/purple/pink/red but not in green shades. Is there any particular reason for this?

30 May 2020 5,834 1 View

Is there a fluorescent dye or any method with which Chitosan can generate fluorescence?

I have generated polyelectrolyte complex in the form of fibers using Chitosan and polygalaturonic acid and in order to observe the fibers under confocal microscope they have to generate fluorescence.

11 March 2020 6,627 4 View

What are some physical methods to determine binding affinity of a protein?

What are various options available while determining the binding affinity of a protein to it's target? In my case the target is also a protein. Are there any standard physical methods to...

09 March 2020 8,053 8 View

Is there a way to convert a 2D matrix into 1D vector which preserves the joint distribution among the columns in the matrix?

I have a matrix where each column represents a feature and each row represent a data point. I am trying to convert this matrix into a 1D vector which can contain the joint distribution among the...

01 March 2020 6,408 3 View

How do I interpret the below test for Uni-variate normality?

Hi, I have a large data sample with 15k rows and I am trying to perform uni-variate normality analysis on the dataset. I have come to understand that normality tests are really sensitive towards...

11 March 2019 3,041 4 View

How to acidify samples when using GC-FID for free Volatile Fatty Acid quantification?

Hi everyone, I use the GC-FID with the StabilWax DA column (http://www.restek.com/chromatogram/view/GC_CH00284) for volatile fatty acid (VFA) quantification (C2 - C7 acids). For this, I use 2%...

15 March 2018 9,752 15 View

What kind of ANOVA to use for my analysis with four factors?

I am doing a study based on the speech measures with gender, health condition, different passages read and different number of sentences from each passage. The number of levels in these factors...

16 January 2018 310 6 View

Curious if desalination plants publish their utilization rates? i.e. how much of their built capacity they produce on a day-to-day basis?

In other words, I am looking for daily/weekly/monthly water production data of all large-scale desalination plants (above 20,000 m3/day).

11 October 2017 6,261 3 View

How to avoid the cloudy appearance of media?

Since last few weeks, I'm facing a problem with my resistant 231 cell line. After every third or fourth passage, the media turns a little turbid in 2-3 days, and I have to thaw a fresh vial. But...

25 May 2017 3,853 4 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

Jerry Alan Warden-Smith

What species are you looking at?

Alejandro Rodríguez

Maybe the RNA in that sample was degraded before retrotranscription? If you still have RNA, you can check its integrity.

is the treatment you are using killing the samples, could there be an error in RNA extraction, is the purity (260/280 ratio) OK?

Deblina Bharadwaj

I am working on breast cancer cell lines. After extraction, the RNA concentration was ok and purity was also good.

Thanks for your answers. My problem is solved now