Jerry Alan Warden-Smith

3 Questions 20 Answers 0 Followers

Questions related from Jerry Alan Warden-Smith

How long should confluent vascular smoth muscle cells in culture be left in serum free medium before starting an experiment?

I am growing human vascular smooth muscle cells for my experiments looking at gene expression with various treatments, and have a question about procedure. Once the cells are confluent I switch...

12 January 2016 2,099 7 View

Has anyone any experience of using alizarin red to quantify calcium content\deposition as well as visualising calcium deposits?

I found a paper published in 2004 (Gregory et al, Anal.Biochem., 329, 77-84, 2004) where they used alizarin red to both visualise and quantify calcium content and deposition. I wondered if anyone...

20 October 2015 5,631 2 View

Can anyone give me some advice about getting started with QIIME software?

I am looking at using QIIME to compare biomes, but finding it difficult to get started as I am not used to command line driven programs. Is their a good beginners guide on the net somewhere, or...

12 September 2014 3,472 5 View