I need antibodies to label PBMC in humans and mice. Thanks.
Hi! I am using a non-parametric bootstrap to calculate SEs and 95% confidence intervals of occupancy values in unmarked in R. I want to infer if the difference between the occupancy value of year...
24 September 2023 3,233 2 View
Estimados, me encuentro realizando una revisión de literatura y necesito incluir bases de datos de literatura gris en español. Tiene algún dato? I am currently conducting a literature review...
20 June 2023 2,489 0 View
We need collagen IV coated plates in order to culture primary queratinocytes. However, the usual supplier of this kind of material (Corning) is not supplying it until, at least, 2024. Does anyone...
23 February 2023 6,995 1 View
I have flow cytometry data of T cells for which I measured the expression of 5 exhaustion markers. I would like to know how many of my cells simultaneously express any combination of 1, 2, 3, 4 or...
17 July 2022 4,584 2 View
I would like to use the SKBR3 cell line in a long term co-culture with engineered T cells. To reduce their proliferative capacity and prevent rapid depletion of the media I would like to irradiate...
06 April 2022 5,533 0 View
The aim is to extract cyclic peptides, alkaloids, diaminoacids, And at the same time get rid of the pigments and phenolic compounds We tried with 80% MeOH in water, but still there are lots of...
24 June 2021 9,355 3 View
We are working with a Malvern Zsizer Nano ZS and the instrument is giving us an error message “the count rate is too low….” in automated measurements. It seems unable to find optimum attenuator...
01 June 2021 3,109 8 View
Hi Everyone! I have a sample of bacteria cells, and I want to sonicate them to extract cytosolic proteins. I found a protocol using Tris-HCl buffer, but this lab did not use proteases inhibitor....
13 December 2020 2,769 3 View
Hi everyone! I am looking for a database that contains a list of organisms, preferentially bacteria, present in the air, water, and soil. Does anyone know any good database/website? Thanks!! Rocio
24 March 2020 2,204 3 View
Why is STZ considered to be inconvenient for type 1 diabetes? if when you inject the rats they are not insulin dependent at low doses (45 mg/kg), but if they are hyperglycemic rats. if it were...
19 March 2020 576 4 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
