Can Flow cytometry data be gated based on the expression of more than two genes at the same time?

More Rocio Castellanos Rueda's questions See All

Why carry out so many studies of associating anthropometrics and health risks if the causal risk appears to be biased in most of them?

Anthropometrically and conceptually, association does not equate causation. However, regarding anthropometric indicators (i.e., metrics of general and abdominal obesity), most of studies worldwide...

20 April 2024 8,779 6 View

¿Cuál es la diferencia entre realidad natural y realidad social con referencia a la producción del conocimiento?

Se trata de establecer los alcances y limites de la investigación en las ciencias sociales, así como la diferencia en métodos con las ciencias naturales para generar hallazgos y producir conocimiento.

07 February 2024 8,490 3 View

How can I perform a qPCR for plants with virus coinfections ?

I need to evaluate viral accumulation by qPCR in plants with DNA and RNA virus coinfections . I dont know how proceed in tge sample treatment before the qPCR . Please someone has done this before ?

04 December 2023 3,651 0 View

Does anyone have advice for adherent cells not attaching to multi-layer flasks when scaling up?

I have been culturing human microvascular endothelial cells (HMEC-1) on my own-made low-serum media. The cells expand fine in wells and flasks T25, T75, and T180 using 0.2 ml/cm2, so the media is...

23 November 2023 1,439 1 View

Reference for using bootstrap as a mean to infer difference significance?

Hi! I am using a non-parametric bootstrap to calculate SEs and 95% confidence intervals of occupancy values in unmarked in R. I want to infer if the difference between the occupancy value of year...

24 September 2023 3,233 2 View

Do the most recent advances in environmental chemistry have the potential to reduce greenhouse gas emissions?

One example of a recent method for carbon capture and storage is chemical reaction carbon capture and storage (CCUS). In this method, carbon dioxide is captured from flue gases and then stored in...

07 September 2023 6,347 4 View

Buscadores de literatura gris en español? - Databases of grey literature in Spanish?

Estimados, me encuentro realizando una revisión de literatura y necesito incluir bases de datos de literatura gris en español. Tiene algún dato? I am currently conducting a literature review...

20 June 2023 2,489 0 View

Collagen IV coated plates for cell culture?

We need collagen IV coated plates in order to culture primary queratinocytes. However, the usual supplier of this kind of material (Corning) is not supplying it until, at least, 2024. Does anyone...

23 February 2023 6,995 1 View

Is there any substitute for the DAX-8 column for the extraction of humic and fulvic acids?

We are looking to separate humic and fulvic acids from compost. We were going to use DAX-8 resin, however it is discontinued. In some papers I have found that they use XAD-7, XAD-1180... but I...

05 February 2023 3,808 5 View

Can anyone give me a suggestion with Gibson Assembly ?

Im running a Gibson Assembly, using two fragments of 2750 pb and a vector of 3300pb. Then when I transform in DH5alfa or 10 beta got a higher band than I expected. Also I did PCR from miniprep and...

03 December 2022 3,356 0 View

Has anyone tried freeze flow samples in -80C freezer or liquid nitrogen before processing?

Our lab has some radioactive cell samples that need to be kept frozen for at least 30 days before flow analysis. For some reason, the samples stored in liquid nitrogen did not perform as well as...

15 July 2024 9,975 1 View

Is this cell cycle analysis correct?

When I applied the cell cycle algorithm to these two samples in FlowJo, I encountered some concerns regarding the accuracy of the fit. The images include both a control and an apoptosis-inducing...

18 June 2024 1,984 0 View

Which of the following proliferation-tracing dyes perform the best?

I'm designing a cytotoxicity assay to measure the effect of treatment on proliferation. My specific objective is to compare the number of cells with 1 and 2 divisions in treated cultures to...

24 April 2024 2,566 0 View

How to generate UMAP plots for my flow cytometry data?

Dear all, I am having an issue with UMAP-FlowJo; I am trying to generate UMAP with my flow cytometry data, although I am following all the steps in the video tutorial with FlowJo, unfortunately,...

06 February 2024 9,553 3 View

What is the optimal method to activate CD4+ T lymphocytes to assess CD107a marker by flow cytometry?

Hi everyone! I am performing an assay to evaluate CD107a production by CD4+ T cells using Flow Cytometry. Currently, I am stimulating a suspension of tonsillar cells with OKT3 only at 37°C for 3...

27 January 2024 6,600 2 View

How to compare MFI if the numbers of cells are low (or 0) compared to the other group?

FLOW CYTOMETRY: I have 2 groups (treated and untreated) in my experiment at different time points. In the untreated group, some cell population counts are expected to become very low (even 0)....

16 October 2023 8,547 0 View

How to plot MFI from FlowJo?

Hi, I need to plot MFI for different channels I used when FACS-characterizing P0-P4 cells. However, I am confused on how to do so. First, I basically selected in "Statistics" in Flow Jo the...

01 September 2023 6,103 1 View

How to import compensation matrix from Kaluza to Flowjo?

Since compensating on Kaluza is easier than Flowjo, I used it. But now I need to put data in Flowjo for analysis. Is there a better way than typing to import compensation matrix made in Kaluza to...

14 June 2023 5,638 3 View

FACS Analysis: What went wrong?

I am a newbie in FACS analysis: so I used this reagent from dojindo called cell cycle blue reagent and do staining in live cells (not like PI) they stated that fixation is not needeed, the problem...

08 June 2023 6,644 6 View

Why does my mouse flow data indicate two population densities for my lymphocytes?

I'm relatively new to flow cytometry work. I harvest mouse samples (blood, lymph node, spleen) and process them for flow analysis: Single cell suspension --> lyse --> stain --> wash X2...

23 May 2023 5,579 2 View

Violetta V. Vlasova

You can use a "Boolean" function in FlowJo to analyze cells expressing two or more markers simultaneously. There is an option "make AND gate" that allows you to do so.

Xiaoting Wu

Hi, I would suggest you to do a UMAP or t-SNE to cluster your T cells and then you can see different subpopulations shows different combination of the gene expression, this can be done simply by flowjo.