What is the best way to extract protein form bacterial cells?

Hi Everyone! I have a sample of bacteria cells, and I want to sonicate them to extract cytosolic proteins. I found a protocol using Tris-HCl buffer, but this lab did not use proteases inhibitor....

13 December 2020 2,603 3 View

Soil, water, and air microbiome?

Hi everyone! I am looking for a database that contains a list of organisms, preferentially bacteria, present in the air, water, and soil. Does anyone know any good database/website? Thanks!! Rocio

24 March 2020 2,009 3 View

Why is STZ considered to be inconvenient for type 1 diabetes?

Why is STZ considered to be inconvenient for type 1 diabetes? if when you inject the rats they are not insulin dependent at low doses (45 mg/kg), but if they are hyperglycemic rats. if it were...

19 March 2020 348 4 View

Does anyone know an updated plant pathogen database?

Hi all, I am working on proteomics, and I am looking for a plant pathogen updated database. Most of them are no longer available. Thank you so much!

15 January 2020 7,157 3 View

Where can i find dataset of cocoa pod to estimate its ripeness?

Community. I'm working in a project focused on cocoa ripeness estimation using image recognition with machine learning tecniques; nowadays, im looking for a dataset of cocoa pods images to...

19 September 2019 5,946 3 View

How education and entrepreneurship is articulated in your country?

In Ecuador, entrepreneurship is in vogue. The country has a legal framework that normalizes the articulation of Education and Entrepreneurship. In addition, Ecuador has the highest rate of Total...

15 July 2019 7,807 6 View

What do you think about the liberation of women in social life?

In these yars wowen are more importat in this sfere of the life

25 February 2019 6,224 6 View

Does thesis supervision qualifies a researcher to become co-author of a paper?

Dear all, I have recently come across several examples in Social Sciences where supervisors become co-authors of published articles by MSc and PhD students. While I understand a supervisor might...

07 October 2018 2,780 94 View

How can I teach astronomy in middle school?

Sometimes astronomy is a lot like a hard subject to teach about in school, but it's also a very important topic to learn

20 February 2018 3,472 5 View

Gene copy number normalization in ChIP-qPCR??

Hi, my question is about normalization data from ChIP-qPCR using the gene copy number. I did ChIP-qPCR for several histone marks on tRNA-Met and tRNA-Arg genes that are in the same chromosome...

31 December 2017 6,977 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

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03 March 2021 5,360 2 View

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02 March 2021 3,060 3 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View