Collagen IV coated plates for cell culture?

24 February 2023 1 7K Report

We need collagen IV coated plates in order to culture primary queratinocytes. However, the usual supplier of this kind of material (Corning) is not supplying it until, at least, 2024. Does anyone know any other supplier that could serve these products as soon as possible in Spain?

Vatamanu Sergiu-Marian

You can actually coate plates yourself !

Badges
Science topic

More Rocio Jarcovsky's questions See All

Reference for using bootstrap as a mean to infer difference significance?

Hi! I am using a non-parametric bootstrap to calculate SEs and 95% confidence intervals of occupancy values in unmarked in R. I want to infer if the difference between the occupancy value of year...

24 September 2023 3,233 2 View

Buscadores de literatura gris en español? - Databases of grey literature in Spanish?

Estimados, me encuentro realizando una revisión de literatura y necesito incluir bases de datos de literatura gris en español. Tiene algún dato? I am currently conducting a literature review...

20 June 2023 2,489 0 View

Can Flow cytometry data be gated based on the expression of more than two genes at the same time?

I have flow cytometry data of T cells for which I measured the expression of 5 exhaustion markers. I would like to know how many of my cells simultaneously express any combination of 1, 2, 3, 4 or...

17 July 2022 4,584 2 View

Hi, I am considering to buy antibodies for flow cytometry from Sino Biological. Does anyone have experience with them ?

I need antibodies to label PBMC in humans and mice. Thanks.

07 July 2022 9,773 0 View

Has anyone done SKBR3 cell line irradiation to reach cell cycle arrest?

I would like to use the SKBR3 cell line in a long term co-culture with engineered T cells. To reduce their proliferative capacity and prevent rapid depletion of the media I would like to irradiate...

06 April 2022 5,533 0 View

Which extractant solution could extract polar and apolar analytes from algae powder?

The aim is to extract cyclic peptides, alkaloids, diaminoacids, And at the same time get rid of the pigments and phenolic compounds We tried with 80% MeOH in water, but still there are lots of...

24 June 2021 9,355 3 View

DLS measurements message "count rate too low..." with more than 10000,00 kcps. How is it possible?

We are working with a Malvern Zsizer Nano ZS and the instrument is giving us an error message “the count rate is too low….” in automated measurements. It seems unable to find optimum attenuator...

01 June 2021 3,109 8 View

What is the best way to extract protein form bacterial cells?

Hi Everyone! I have a sample of bacteria cells, and I want to sonicate them to extract cytosolic proteins. I found a protocol using Tris-HCl buffer, but this lab did not use proteases inhibitor....

13 December 2020 2,769 3 View

Soil, water, and air microbiome?

Hi everyone! I am looking for a database that contains a list of organisms, preferentially bacteria, present in the air, water, and soil. Does anyone know any good database/website? Thanks!! Rocio

24 March 2020 2,204 3 View

Why is STZ considered to be inconvenient for type 1 diabetes?

Why is STZ considered to be inconvenient for type 1 diabetes? if when you inject the rats they are not insulin dependent at low doses (45 mg/kg), but if they are hyperglycemic rats. if it were...

19 March 2020 576 4 View

Adhesion strength of coating?

How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...

10 August 2024 942 3 View

How do I conformally do PR spin coating on trench structure?

I did PR spin coating on trench structure. I used AZ P4620 PR and the thickness or PR is around 11um. The substrate is Si. And my trench structure depth is 141um(negative way). Even though I...

08 August 2024 2,298 0 View

Why do i have a weak plasma and no coating during rf sputtering?

I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.

07 August 2024 4,526 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

What is the reason I get 400nm nanoparticles using an extruder with 200 nm membrane for cell membrane coating?

I am trying to coat my micelles and PLGA based nanoparticles with cell membrane using an Avanti mini extruder according to literature . I use 200nm membrane but my particles end up to be 400nm And...

30 July 2024 3,294 3 View

What is the main used curing agent for epoxy based coating?

I am trying to coating metal oxide into steel substrate, for better sticking planning to utilse epoxy based, Could you please suggest suitable curing agent for the coating purpose

25 July 2024 438 3 View

Comparison of Methanol and ethanol treatment on collagen based scaffolds?

Kindly share a research paper that explains the effect of Methanol and ethanol treatment on collagen based scaffolds. How it increases porosity and swelling. Kindly help me find a paper. Thank you

24 July 2024 3,937 0 View

How do you get pure primary macrophage cell culture?

I have been harvesting monocyte-derived macrophages from buffalo and cattle fresh whole blood using the same protocol for years and getting good macrophage cultures. Below is the protocol that I...

23 July 2024 4,074 2 View

Fixation Problem with cancer cells?

Hello everyone, I work with polyacrylamide hydrogels, which I fictionalise with sulfo-sanpa and collagen I to then plate lung cancer cells. The problem is that I lose a significant amount of...

19 July 2024 3,906 0 View

What is the suitable solvent of MIL-100 for chemotherapy?

I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...

19 July 2024 8,522 0 View