I am also looking for a annexin-v kit for adherent cells without detach that could be observed under fluorescence microscope directly. However, someone said if you use annexin-v to detect adherent cell the confocal laser microscope have to be used not common fluorescence microscope.
So I want to know did you guys work well or not and could you send me the name of kit and protocol.
Hi Shujun, the protocol is included in the link below my question. But it does state using an ordinary fluorescence microscope not a confocal one !
Hi Cassio, Sorry for the belated reply. I have no experience with using a fluorescence microscope and it's my first time to do the experiment this week. I've just received the kit last week :/. I'll try first on cisplatin treated cells not my drug . Do you have any tips regarding the procedure and using the microscope?