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Questions related from Shujun Zhang
I use lentivirus to deliver shRNA to knockdown my target genes. After 48h post transduction, i use puromycin to select for 24h and then rest cells for another 24h. Then, PMA (100ng/ml) was added...
24 July 2016 4,692 3 View
Hi all I use a compound to induce cell death and found it could be enhanced by zVAD-FMK. So we could exclude the apoptosis and proptosis and considered it might be necroptosis. However,...
11 April 2016 7,412 8 View
Hi everyone, Some papers showed LPS (100-200ng/ml) can induce apoptosis of THP1 monocytes (proved by TUNEL, Caspae 3/7 activity and LDH release). But I couldn't reproduce same results even use...
25 March 2016 535 4 View
I want to isolate and purify Kupffer cells from human with MACS CD14 magnetic beads. Next, we plan to stimulate isolated Kupffer cells with LPS. My question is whether cd14 beads will influence...
17 February 2016 2,177 3 View
I treat THP1 with PMA (100ng/ml) for 24h and then use IFNr (50g/ml for 24h) for M1 polarization and IL4 (40g/ml for 24h) for M2. Because I want to use LPS to stimulate it to induce apoptosis...
09 October 2015 9,496 12 View
We will use EGFP-vector as interfering factor. Both EGFP and FITC have similar excitation and emission, so Vybrant Phagocytosis Assay Kit and some other FITC-labeled microparticles or flow...
04 June 2015 8,875 2 View
I stimulate THP-1 macrophage with LPS (10ng/ml-1000ng/ml) for 24h, but detect no IL-1beta and IL-12 in supernatant. Someone said LPS just increase pro-IL-1beta expression inside cells since...
17 May 2015 7,569 15 View