Shujun Zhang

9 Questions 23 Answers 0 Followers

Questions related from Shujun Zhang

Anyone has experience about using PMA to differentiate THP1 after lentivirus infection?

I use lentivirus to deliver shRNA to knockdown my target genes. After 48h post transduction, i use puromycin to select for 24h and then rest cells for another 24h. Then, PMA (100ng/ml) was added...

24 July 2016 4,723 3 View

How to explain the results that knockdown of TF can increase gene expression for 40 fold but overexpression has no impact on it?

I did overexpression (Roche Xtremegene) and knockdown (shRNA) of one transcription factor in Hela cells and harvest cells for RT-PCR after 48h. Now we can see that knockdown of TF can induce...

25 April 2016 5,944 4 View

Cell death was enhanced by zVAD-FMK and necrostatin-1 has no effect of it. Is it autophagy?

Hi all I use a compound to induce cell death and found it could be enhanced by zVAD-FMK. So we could exclude the apoptosis and proptosis and considered it might be necroptosis. However,...

11 April 2016 7,440 8 View

Dose anyone stimulate THP-1 monocytes with LPS to induce apoptosis?

Hi everyone, Some papers showed LPS (100-200ng/ml) can induce apoptosis of THP1 monocytes (proved by TUNEL, Caspae 3/7 activity and LDH release). But I couldn't reproduce same results even use...

25 March 2016 566 4 View

Can MACS CD14 beads be used to purify human Kupffer cells that will be stimulated with LPS later?

I want to isolate and purify Kupffer cells from human with MACS CD14 magnetic beads. Next, we plan to stimulate isolated Kupffer cells with LPS. My question is whether cd14 beads will influence...

17 February 2016 2,210 3 View

Anybody knows TRAIL-sensitive human cell lines?

Anybody knows which human cell line is sensitive to TRAIL? The more common the better. Thank you!

26 January 2016 4,438 2 View

How can I induce polarization (M1 and M2) of THP-1 macrophage and which marker should I test?

I treat THP1 with PMA (100ng/ml) for 24h and then use IFNr (50g/ml for 24h) for M1 polarization and IL4 (40g/ml for 24h) for M2. Because I want to use LPS to stimulate it to induce apoptosis...

09 October 2015 9,539 12 View

can anybody recommend a method for phagocytosis assay of THP-1 macrophage?

We will use EGFP-vector as interfering factor. Both EGFP and FITC have similar excitation and emission, so Vybrant Phagocytosis Assay Kit and some other FITC-labeled microparticles or flow...

04 June 2015 8,903 2 View

Does anyone stimulate THP-1 with LPS to detect IL-1beta and IL-12 in supernatant?

I stimulate THP-1 macrophage with LPS (10ng/ml-1000ng/ml) for 24h, but detect no IL-1beta and IL-12 in supernatant. Someone said LPS just increase pro-IL-1beta expression inside cells since...

17 May 2015 7,610 15 View