Hi can I leave PBMC culture for 8-10 hrs after addition of BFA?

Arshi Munawwar @Arshi-Munawwar

19 May 2016 1 3K Report

I added PMA 100 ng/ml and Ionomycin 1 ug/ml after two hours of incubation I added 1/1000 BFA to my culture.

Andre Luiz Zorzetto-Fernandes

Hi Arshi

Yes you can. Just don't keep BFA for more than 12h because it becomes toxic to your cell.

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