23 February 2022 1 1K Report

We are trying to do some slice cultures and we need an established protocol for cultures. Also how transduce the slices with AAVs ?

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How to get links for copyrights for papers?

how to get links for copyrights for papers?

06 August 2024 7,410 1 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 841 2 View

How to perform band structure calculation of a bulk, nonmagnetic superconductor with spin-orbit coupling using vasp?

I'm new to VASP and I'm trying to calculate the band structure of a bulk, nonmagnetic superconductor with a tetragonal crystal structure. I'm including spin-orbit coupling (SOC) in my...

09 July 2024 5,641 3 View

Do social development programs integrate women into climate change adaptation initiatives, especially within the framework of poverty reduction?

Gender, along with socio-economic and environmental factors, is a key determinant of vulnerability to climate change.

24 June 2024 8,437 2 View

Why the number of atoms is different in xyz file and POSCAR file?

am trying to make POSCAR file and xyz file using vasp for NbReSi compound using VESTA. However, I notice that the number of atoms for xyz file is given as 41 whereas for POSCAR is it 36. Can...

17 April 2024 698 2 View

Alternative to nitrogenase?

I have seen nitrogen fixation in three heterotrophs, but cannot detect any nitrogenase in their genomes. Are there any alternative pathways that I can look into that may provide information about...

01 April 2024 5,641 0 View

XRD peak deviation?

In XRD analysis, when we move to a higher angle, like above 50 degrees, if I have two reference lines of different phases, one matches the peak and the other is towards the right of the peak with...

31 March 2024 1,812 2 View

What would be a standard protocol for termite sampling in sub-tropical Himalayan foothill forests?

My study area is in eastern Himalayan foothill landscape which is a tropical to sub-tropical forest; elevation ranges from 150 to 1300 meter. The forest is some parts dominated by sal, tick and...

21 March 2024 8,297 1 View

Help regarding Geometry Optimization of a bimetallic surface?

I am trying to perform a geometry optimization (GEO_OPT) of a FCC 111 Pt_{0.5}Au_{0.5} bimetallic surface (10x10x3) generated from ASE, with a lattice parameter of 3.9 A∘A∘ in CP2K. However, after...

18 March 2024 3,328 2 View

Which one of the following showing the correct structure Jsmol or Vesta?

Let me explain. I found that the structure for TiFeSi given in the ICSD database using Jsmol software is a little different from what can be obtained using Vesta software using the same cif file...

19 February 2024 9,722 1 View

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Good Day, can I ask for your help on the questionnaire that I want to adopt?

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Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

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