Hi Inas, I think PBS should be fine since you need to wash away serum before trypsin anyway.  Good luck!

Thanks a lot for your feedback.

Hi,

Some media contain trypsin inhibitor, so I would suggest using PBS.

Thanks a lot for the precious tip.

If you dilute it in PBS, make sure you use sterile PBS, without Mg2+ / Ca2+. Mg2+ and Ca2+ are trypsin inhibitors.

Thanks a lot Alexander, that is absolutely right, I did it and worked well. Appreciate your help.

how long you can save this 0.05% trypsin? Please reply

I used to prepare it fresh. Not sure if this is the best way or not, but I was not sure if it was stable enough or not.

ok thanks, Inas. I'll prepare fresh too.