What the Scientific reason that cause fast spreading of corona virus through short time?

Despite there are many viruses, bacteria, germs, nanomaterial in atmosphere, but corona virus can be spreading fastly

29 December 2020 3,800 9 View

What is the best media/solution for preservation or transfer of samples from the gingiva for bacterial identification and PCR? is TE buffer enough?

I have read many researchers used VGMA III and Stuart media but we don't have these media in the lab right now and would like to process my samples quickly. would appreciate your advice regarding...

11 March 2018 9,244 3 View

Carboxylic acids..chemists supposed to be here?

19 March 2016 8,123 8 View

Can anyone help with transfecting immortalized cornea epithelial cells with miRNA mimic using lipfectamine?

Hi All, Do you add the lipofectamine miRNA mix to the media which the cells are already in or remove the media and just add the lipfectamine-mimic mix in OPti-MEM media. And I also need to know...

23 June 2015 1,174 2 View

What method is most suitable for water annealing for film preparation using a desiccator?

What is the main idea of water annealing process on film formation by using desiccator? In your opinion is water or methanol the best one for increasing insolubility?

03 March 2015 7,979 3 View

Why is there is no peak when dissolving hederagenin in methanol by HPLC?

Best method for hederagenin saponin analysis on HPLC/UV.

09 January 2014 954 4 View

How to reduce debris with neuron isolation?

Hi there, I'm isolating neurons from PND1 guinea pigs and have noticed a bit of debris in the early days (this is day 4 plated) and a lot of variability between plates. Current protocol is...

14 February 2021 3,692 4 View

How to improve NHBE adhesion after subculture?

I'm trying to subculture NHBE cells from Lonza, however, the cells seem to not adhere to the new flasks after detachment. I've followed Lonza detachment protocol (2 mL/25 cm2 of Trypsin 0.05% and...

10 February 2021 196 1 View

MS/MS protein analysis?

Hi Everyone: I want to quantify the relative amount of a protein in 2 samples. I will first digest the sample with trypsin, and subject the resulting peptides to LC-MS/MS analysis. I will then...

25 January 2021 8,333 3 View

FLICA assay not working?

Hi everyone, I am trying to perform the FLICA apoptosis assay with my MSCs. However, although the cells look healthy upon inspection and the viability is greater than 90% with cell counting, the...

17 January 2021 6,959 1 View

How to optimize cell suspension protocol for flow in LS-180 cell line?

Hi, I am optimizing a flow protocol for LS-180 cell line, which can not be trypsinized. First experiment wend suboptimal due to detritus and doublets Any trips or tricks for making this cell...

06 January 2021 6,117 2 View

What are these black and white spheres in T25 flask?

I just thawed my 4T1 cancer cells and 24 hours after seeding the cells in T25 flask there were large black and white spheres (approximately 1000times bigger than my cells) in my cell...

18 December 2020 3,302 3 View

Can I fix the cells after H2DCFDA assay for FACS analysis?

Hi ! I'm Yu, and planning to analyze ROS assay with H2DCFDA(Thermo) on MSCs. A lot of protocols instruct that treat H2DCFDA at specific concentration for 5~30 min, 20~30 min of recovery time...

10 December 2020 973 1 View

Do I need collagenase to dissociate hepatocytes?

Hi all, after culturing hepatocytes in a monolayer on collagen in 24 well plates for 5 days, I attempted to trypsinize (0.25% trypsin) them to perform a cell count. However, none of my cells...

11 November 2020 2,478 2 View

Any expert on Calu-3 cell culture?

I just started working months ago with this peculiar cell line and I still cannot figure out the doubling time or passaging. Some times take 72h to see double amount of seeded cells, some times...

23 October 2020 6,151 2 View

Currently i am culturing Influenza A in MDCK cell line and i wanted to ask is it possible to obtain high titre of virus without adding TPCK trypsin?

I have done 3 blind passages to concentrate the viral stock in the supernatant without adding TPCK Trypsin. After passage 1 my Ct value for INF A was 36.96. After P2 the value decreased to 33.36....

21 October 2020 3,949 3 View