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Questions related from Alexander Streng
Units to indicate the enzyme activity of thrombin seem to deviate from the "standard" units. How do I convert the NIH - units to micromoles / minute?
08 August 2016 9,191 2 View
What are normal concentrations of thrombin in human serum? Are these concentrations representative for the normal thrombin concentration in blood?
08 August 2016 6,374 4 View
We want to detect phosphorylated proteins using the ProQ Diamond (life technologies) and Sypro Ruby (Bio-Rad) stains. PeppermintStick phosphorylated protein standard was used to test this. We...
10 April 2015 2,415 1 View
During sample preparation for mass spectrometry, disulfide bonds are reduced with DTT followed by alkylation of the free thiol group on cysteines. But if your protein of interest does not contain...
18 December 2014 7,930 5 View
This is what I did: I serially diluted my protein of interest in human serum. Concentrations were measured with an immunoassay. The protein was then captured using immunoprecipitation. Eluates...
28 November 2014 4,635 2 View
Hello all, I am setting up a method to relatively quantify several proteins of interest on the Q-Exactive. I am in doubt of which method to use. The first option is t-SIM with a dd-MS2 for peak...
02 October 2014 2,963 8 View
I am interested in phosphorylating my protein on specific sites. Are there any online tools to select a kinase that will specifically phosphorylate the sites of interest on my protein? What other...
26 June 2014 2,343 4 View
I want to calculate the area under the curve of the extracted ion chromatograms of my mass spectrometry dataset using Proteome Discoverer (Thermo Fisher). However, the area shows as zero for all...
17 April 2014 8,333 5 View
We have a working immunoprecipitation protocol for my protein of interest, which has recoveries over 90% when using small volumes of serum (200-500 uL). We now want to scale up and...
14 November 2013 1,639 5 View
