Inas Helwa

3 Questions 7 Answers 0 Followers

Questions related from Inas Helwa

I am getting extremely low counts of Droplets with Digital PCR (Biorad), Any clue why? Any tricks?

My Droplets Count range is between 1000-6000

24 July 2015 3,280 7 View

Can anyone help with transfecting immortalized cornea epithelial cells with miRNA mimic using lipfectamine?

Hi All, Do you add the lipofectamine miRNA mix to the media which the cells are already in or remove the media and just add the lipfectamine-mimic mix in OPti-MEM media. And I also need to know...

24 June 2015 1,251 2 View

Hi All, What would I use to dilute trypsin from 0.25% to 0.05% for splitting? Should I use sterile PBS or serum free media?

I am using Cornea epithelial cells from ATCC

08 June 2015 8,398 9 View