Priti Gupta

6 Questions 9 Answers 0 Followers

Questions related from Priti Gupta

How many mouse splenocytes were stored in each cryovials?

I am planning to cryopreserved mice splenocytes in 10% DMSO +90% FBS media ( 1ml is added in each vial) for future experiments. I am NOT sure what's minimum number of mice splenocytes cells should...

04 December 2018 5,875 1 View

Can we perform Flow cytometry based NK cell cytotoxicity assay using melanoma cell line (B16F10)?

I did Flow Cytometry based NK cell cytotoxicity assay using anti-CD71 targets K562 and U266 cell line. Now I want to repeat same experiment using melanoma cell line (B16F10). Would you please...

07 December 2017 1,820 3 View

Should freeze down mice splenocyte before the RBC lysis or after?

I am planning to perform CD107a degranulation assay on frozen splenocyte. Should I store splenocyte before RBC lysis buffer OR after lysis.

01 January 1970 1,085 3 View

CD107a degranulation assay in mice- NK cells?

I am planning to do CD107a degranulation assay and intracellular staining (IFNy) in mice splenocytes. So, for assay, I am planning to have a total of 5-hour incubation adds (will add Monesin after...

01 January 1970 3,574 3 View

CD107a degranulation assay in mice- NK cells

Hello All, I am planning to do CD107a degranulation assay. Would you please recommend me what concentration of phorbol-12-myristate-13-acetate (PMA) and ionomycin will be good for assay (Positive...

01 January 1970 4,480 4 View

Worthington Collagenase and DNAse expiry date

Hello All. I purchased collagenase (type I) and DNAaseI from Worthington enzymes in early 2018. There was no description of expiration date on the product. Would you please let me know how long...

01 January 1970 3,969 2 View