Who experience on SDS-PAGE with a small peptide?

Is there anyone who has experience on SDS-PAGE with a small peptide (MW =~ 1- 2 kDa)? Is anyone able to offer advice on techniques?

03 January 2021 4,424 3 View

What the Scientific reason that cause fast spreading of corona virus through short time?

Despite there are many viruses, bacteria, germs, nanomaterial in atmosphere, but corona virus can be spreading fastly

29 December 2020 3,800 9 View

What is the best media/solution for preservation or transfer of samples from the gingiva for bacterial identification and PCR? is TE buffer enough?

I have read many researchers used VGMA III and Stuart media but we don't have these media in the lab right now and would like to process my samples quickly. would appreciate your advice regarding...

11 March 2018 9,244 3 View

How long oligopeptites can be precipitation by TCA ?

Proteins can be precipitated by TCA, can tripeptides, pentapeptides, hekzapeptides and other oligopeptites can be precipitated by TCA ? What is the amino acid limit ?

23 April 2016 8,114 3 View

Carboxylic acids..chemists supposed to be here?

19 March 2016 8,123 8 View

Can anyone help with transfecting immortalized cornea epithelial cells with miRNA mimic using lipfectamine?

Hi All, Do you add the lipofectamine miRNA mix to the media which the cells are already in or remove the media and just add the lipfectamine-mimic mix in OPti-MEM media. And I also need to know...

23 June 2015 1,174 2 View

How can we distinguish transconjugant cells from recipient cells in minimal agar medium?

03 December 2014 7,892 4 View

Why is there is no peak when dissolving hederagenin in methanol by HPLC?

Best method for hederagenin saponin analysis on HPLC/UV.

09 January 2014 954 4 View

Problem in detection of a specific gene & elimination of primer dimer in qpcr?

In our lab, we are trying to do real time PCR for some genes. One of them has a size of 85 bp. Whatever we do, we had an amplification in negative control. When we run agarose gel, it always a...

14 February 2021 9,750 11 View

Why non-specific band appear on T-ARMS PCR?

Hi everyone. We want to know how can I remove the non-specific band amplification on this variant. Although the amplification of non-specific band is not as intense as the band that we want, but...

09 February 2021 588 2 View

I have faced a problem to amplify VP2 gene of porcine parvovirus 1,2,3 and 5? Please suggest me how can i solve this problem?

Till now i haven't got any PCR amplified vp2 gene bands on specific annealing temperature and in step up PCR.

08 February 2021 819 4 View

Xrd result of ZnO thin film shown better crystallinity at low temperature and the crystallinity decreases as the temperature increased. why?

I have synthesized ZnO thin films at four different substrate temperature say, 300, 350, 400 and 450C. and the samples were annealed at 450C for half an hour. the xrd result shown better...

04 February 2021 7,641 4 View

How can I partially remove the metal oxide layer for solar cell device?

Hi, I want to make a perovskite-based planar solar cell device. (electrode/HTL/perovskite/ETL/FTO) To fabricate the device, one electrode should be deposited on HTL and the other should be...

01 February 2021 229 6 View

Are there any technical and safety tips or details for hot salt baths?

29 January 2021 6,036 2 View

Do you have experience of second round PCR amplification issue?

When 1st-round PCR amplification was finished , I could get a clear expected band, but the amount was very low, so I cut the band and run second-round PCR with same condition, it didn't work, I...

21 January 2021 888 4 View

Sb2Te3 thin film annealing and damage(break)?

21 January 2021 9,642 2 View

Strategies for short hairpin RNA interference cassettes?

In your experience, how much does the distance between the U6 promoter and the annealed -short hairpin - oligonucleotides affect the RNAi cassette expression? How to clone a shRNA sequence...

16 January 2021 9,418 2 View

I am trying to amplify my gene of interest (GOI, 2000 kb long) by PCR. My previously designed primers are not working. How can I optimize the primers?

As I wanted to amplify my GOI, I chose first 18 nucleotides including start codon in my forward primer. I add BAMHI recognition site at the beginning and 3 nucleotide extension for the efficient...

12 January 2021 3,327 3 View