So I am trying to do ISH on adult drosophila testis and ovaries. So far I have the procedure down until the last part on day 3 where I am doing the developing/enzymatic reaction and the mounting. Currently the protocol calls for me to transfer from a tube into a dissecting disc well. I can do this transfer fine. The next step is adding the reaction solution, waiting, then washing with a few different things, and afterwards adding GMM (Canada balsam:methyl salicylate) to the wells and then taking that and putting it on a slide to mount. My difficulty is that the testis and ovaries are sticking to the glass in the dissecting well and when i try to dislodge them many end up disintegrating. As such from starting with 50 in a well i ended up with 3 on the slide. I am hoping to increase the success rate on this. 

To that end I think I have a solution and would like to run it all by the community here for feedback and suggestions. I am proposing taking a poly lysine coated slide and using a grease/wax pen to draw a barrier and make a mini well. I would then add the testis directly into this mini well and perform all the reaction and washes within it. My reasoning is that since my main problem is that the testis are sticking to the glass and disintegrate on contact of when being disturbed. If i put them directly on the slide I should be able to use this weakness as a strength. What is everyones thoughts on this?