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Questions related from Andrew N Gale
I am currently trying to prepare a library for illumine deep sequening using the yeast Candida glabrata. Ive been having difficulty generating a high quality library. My collaborators believe that...
28 November 2016 1,097 1 View
I am trying to create a genomic library for transposon integration profiling in yeast. Recently I have been getting a very strange fragmentation pattern. It seems that my fragmentation is at the...
31 October 2016 232 3 View
So I am trying to do ISH on adult drosophila testis and ovaries. So far I have the procedure down until the last part on day 3 where I am doing the developing/enzymatic reaction and the mounting....
28 June 2016 3,364 3 View
Hello, I am working with imaging in larval zebrafish. I am interested in looking into the expression patterns of a particular protein in real time along their intestine in response to lipid...
23 September 2015 4,157 2 View
I am trying to do KASP genotyping using a cfx96 Biorad qPcr. When i tried my first sample the rfu of the negative control, water, was just as strong as my samples and the controls. Later I tried...
11 August 2015 1,027 3 View
I am trying to decide what type of florescent protein to use for a plasmid. The mRNA levels are rather strong for this protein and I am building a reporter system to cause a florescent protein to...
13 July 2015 3,390 1 View
I am trying to find a pair of potential enzymes that would create compatible sticky ends for a protein fusion. I am very limited for enzymes that I can use. is there any site or program that I can...
12 July 2015 4,573 3 View
