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Questions related from Daniela Orquera
So, I'm about to start this new project and I'm wondering if there are new trending techniques to insert LoxP sites flanking an exon in the mouse genome. We use CrispR/Cas9 system in the lab, so...
28 June 2016 4,784 2 View
What are your experiences with in situ PCR vs ISH? Is the detection threshold better for in situ PCR? What kind of equipment do I need for in situ PCR? I've read a thermocycler but... what kind of...
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Does anyone prepare big master mix batches (that means primers, buffer, magnesium chloride, dNTPs, water; so everything except for the Taq) to final concentration for genotyping purposes? What I...
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I'm trying to make some DIG-labelled probes work, and I am not sure where to start trying to optimize the protocol. I'm doing ISHES on e12,5 mouse embryos, fixed ON in PFA 4%. My issue is that I...
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I need to perform a ChIP on chip assay, and I need a custom made array of a specific locus, and I'm totally new and looking for information on the subject. Does anyone have experience with this?...
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I've been working with the CreER System for the past three years. About 10 months ago, I noticed the recombination in my animals was not 100%. The past couple of months it's not even working. My...
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