Daniela Orquera

6 Questions 17 Answers 0 Followers

Questions related from Daniela Orquera

Inserting LoxP sites in the mouse genome?

So, I'm about to start this new project and I'm wondering if there are new trending techniques to insert LoxP sites flanking an exon in the mouse genome. We use CrispR/Cas9 system in the lab, so...

28 June 2016 4,789 2 View

In situ PCR vs ISH?

What are your experiences with in situ PCR vs ISH? Is the detection threshold better for in situ PCR? What kind of equipment do I need for in situ PCR? I've read a thermocycler but... what kind of...

24 May 2016 8,336 3 View

PCR master mix for genotyping purposes?

Does anyone prepare big master mix batches (that means primers, buffer, magnesium chloride, dNTPs, water; so everything except for the Taq) to final concentration for genotyping purposes? What I...

09 March 2016 7,011 4 View

Probe optimization in DIG-labelled In situ hybridization?

I'm trying to make some DIG-labelled probes work, and I am not sure where to start trying to optimize the protocol. I'm doing ISHES on e12,5 mouse embryos, fixed ON in PFA 4%. My issue is that I...

17 September 2014 3,365 5 View

ChIP-Chip. Best company to have a custom-made array?

I need to perform a ChIP on chip assay, and I need a custom made array of a specific locus, and I'm totally new and looking for information on the subject. Does anyone have experience with this?...

04 September 2013 6,073 1 View

TAM injections in the CreER-LoxP system?

I've been working with the CreER System for the past three years. About 10 months ago, I noticed the recombination in my animals was not 100%. The past couple of months it's not even working. My...

21 August 2013 3,880 26 View