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I'm a newcomer to this field and I'm trying to understand the whole CADD workflow. I've been reading reviews nonstop and I know the theory behind it, but I have no idea where to start. I do not...
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So, I'm about to start this new project and I'm wondering if there are new trending techniques to insert LoxP sites flanking an exon in the mouse genome. We use CrispR/Cas9 system in the lab, so...
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Does anyone prepare big master mix batches (that means primers, buffer, magnesium chloride, dNTPs, water; so everything except for the Taq) to final concentration for genotyping purposes? What I...
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I'm trying to make some DIG-labelled probes work, and I am not sure where to start trying to optimize the protocol. I'm doing ISHES on e12,5 mouse embryos, fixed ON in PFA 4%. My issue is that I...
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I need to perform a ChIP on chip assay, and I need a custom made array of a specific locus, and I'm totally new and looking for information on the subject. Does anyone have experience with this?...
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I've been working with the CreER System for the past three years. About 10 months ago, I noticed the recombination in my animals was not 100%. The past couple of months it's not even working. My...
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Has anyone had trouble with pSMAD-1 antibody when using PBS buffer to wash the sections? I've been told other antibodies against phosphorilated proteins require the use of TBS instead of PBS. I'm...
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