Hello, I want to compare some viability methods in one cancer cell line after irradiation, including MTT , Trypan blue and colony forming assay?

More Fahime Faqihi's questions See All

Why Non- Permeabilized cells show the nuclear staining?

I am using Immunostaining to evaluate a cell surface expression of one protein. when I use non-permeablization technique to just stain the cell surface localization (fixed cells with PFA , 2% 5...

31 December 2018 1,363 1 View

Hi there, what is the best way for culturing normal brain cell in form of spheroids?

I used liquid overlay tech for glioblastoma spheroids , but I am not sure that it works for astrocytes . do you have any experience with this method or other ones for normal cells

07 August 2016 4,844 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Krishnanand Mishra

Dear Faqihi

MTT is a measure of metabolic activitylviability of cells and might not represents/reflects the total cell death. The metabolic activity may increase or decrease depnding upon radiation dose, cell type etc. Trypan blue is a tricky process if you are not doing it carefully, it will give total cell death including apoptosis, if performed before 24 hr. Colony forming assay provides total cell death that includes mitotic, apoptotic, necrotic etc. You can't measure cell death in any assay more than colony forming. So its ultimate parameters of cell death but time taking and tidious.

regards

Krishna