Fahime Faqihi

3 Questions 4 Answers 0 Followers

Questions related from Fahime Faqihi

Why Non- Permeabilized cells show the nuclear staining?

I am using Immunostaining to evaluate a cell surface expression of one protein. when I use non-permeablization technique to just stain the cell surface localization (fixed cells with PFA , 2% 5...

01 January 2019 1,389 1 View

Hello, I want to compare some viability methods in one cancer cell line after irradiation, including MTT , Trypan blue and colony forming assay?

Can I infer type of death from these methods? I mean that how can I explain the differences between results from these three techniques? Your answer is really appreciated

12 December 2016 4,374 2 View

Hi there, what is the best way for culturing normal brain cell in form of spheroids?

I used liquid overlay tech for glioblastoma spheroids , but I am not sure that it works for astrocytes . do you have any experience with this method or other ones for normal cells

08 August 2016 4,866 2 View