Hello everyone,
I extracted genomic DNA from electroplated HEK293 cells using QIAGEN blood & tissue kit. Unlike my expectation that the extracted genomic DNA would be at least 50kb in length, electrophoresis revealed clear band size of approx. 19kb. I feel that the size 19kb band indicates bizarre result because it is too small compared to what genomic DNA of human cell should be (very large, >50kb)? What am I missing here?
Any suggestions would be greatly appreciated!
Best regards,
Maybe it is sheared. How did you figure out it was 19 kb-s? You got a gel picture?
Leonid,
I think shearing is possible,
and no I don't have gel picture but for reference, I found this attached image from online source which shows gel image of HEK 293 genomic DNA. This gives me an idea that HEK293 total extracted genomic DNA could be a tad bit larger than 10kb, well, at least from the provided gel image combined with my own experience of electrophoresis results.
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Biology
- Molecular Methods
- Cloning