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Hello everyone, I extracted genomic DNA from electroplated HEK293 cells using QIAGEN blood & tissue kit. Unlike my expectation that the extracted genomic DNA would be at least 50kb in length,...
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Hello everyone, I am trying to carry out my experiment in order to insert multiple exogenous genes into a common vector pUC19, as described below: 1. insert gene 'A' at site 'A' in pUC19-->...
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Hello, I performed midiprep in order to purify plasmid DNA from Ecoli DH5-alpha and obtained 72ug of the plasmid DNA. Nanodrop results showed good 260/280 ratio of 1.9, indicating a fair purity...
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I am trying to detect expression of gene of interest by constructing a multicistronic vector that incorporates 'P2A' and 'tdTomato', so basically, modified gene would look something like: [gene of...
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