How to seperate PEG-4000 from aqueuse phase ?

Hello everyone , Please tell me if It possible to seperat POlyethelyn glycol -4000 from an aqueuse media by other ways rather heating . I have tried salting out by electrolyte solution to...

22 February 2021 7,285 5 View

Is this right way to get Modulation Transfer Function(MTF)?

Hi I'm trying to make a python script to get MTF curve for the input image Here is my algorithm to get MTF. 1. get Image 2. select ROI(High contrast region) 3. extract a center line out of image...

15 August 2020 6,646 3 View

How can I use with ECMWF wind and pressure in MIKE 21 HD modelling and having sinusoidal curve of surface elevation?

I have ECMWF ERA-5 wind and pressure data in NetCDF file, and i was able to convert the data to dsf2 file for use in MIKE 21 HD model. The problem that the results of the simulation (surface...

19 November 2019 3,850 3 View

What is the solution to the error while running MaxQuant ?

Dear all, I use label-free QTOF technique (Bruker) in my project. I got a good result at the first time I analyzed .d folder using MaxQuant version 1.6.5. However, in the last 2 months, error...

18 August 2019 5,009 6 View

ABAQUS:How to solve "ELEMENT is PARALLEL WITH ITS BEAM SECTION AXIS" ?

ABAQUS ERROR: ONE OF THE ELEMENT IS CLOSE TO PARALLEL WITH ITS BEAM SECTION AXIS, so I'd like to know how to solve this problem? The element property is beam element, so I should define the...

08 August 2019 2,092 2 View

Can anyone help me to find a rapid sensitive assay for quantitative methylation analysis ?

Hello all, in my research I need to do quantitative methylation analysis on 3 candidate genes and have to do it in only 4 months, can you please help to find a fast but sensitive method for that?...

19 April 2019 7,728 3 View

Good 260/280 number (1.9), but low individual numbers?

Hello, I performed midiprep in order to purify plasmid DNA from Ecoli DH5-alpha and obtained 72ug of the plasmid DNA. Nanodrop results showed good 260/280 ratio of 1.9, indicating a fair purity...

01 April 2018 1,790 4 View

How to achieve uniform thickness reduction by electrochemical etching?

Hello, everyone! I want to reduce the thickness of copper foil (from 15um to 3um) by electrochemical etching (CuSO4 as electrolyte, lead and copper foil as electrode material). The edge of copper...

27 February 2018 763 4 View

How can I find temperature dependent properties of a material(for example steel)?

I want to simulate a heat treatment with Abaqus. so I need a high range of temperature date of material. thank you.

10 December 2017 5,310 4 View

How to measure collinearity, categorical variable and not normal distribution in SPSS?

Hi! I would like to ask the following: I have between other independent variables severity: severe, high tisk, medium risk, low risk and no risk; and the variable outcome: discharged, death,...

23 November 2017 8,149 5 View

Logistical regression using SPSS for a dichotomous dependent variable, with an independent variable of 1, 2, 5 and 10 seconds?

I have an experiment with 28 participants The independent variable is the times 1, 2, 5, and 10 used as the cross hairs during a task These 4 times were tested using 4 different blocks, each...

03 March 2021 9,692 2 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Can anyone provide the Support vector regression code structure of LIBSVM MATLAB or python ?

Please, if Gaussian Parameter optimization is possible in this code structure

28 February 2021 278 3 View