Hello everyone, I am trying to detect hbx and core protein with western blot, is it better to use a 10% or 12% Sds-polyacrylamide gel? Thank you in advance
12-15 % is the best concentration for these size of proteins. I advise the 12% , because I imagine that you'll detect some loading of protein (a-tubulin, gapdh, actin) , which usually has more than 45 kDa.