8 Questions 19 Answers 0 Followers
Questions related from Iskander Madhi
Hello everyone, Huh-7 cell line can be used to study the HCV replication, but it is not clear if HBV replication is possible too in this kind of cells. Specifically, I am wondering if there's...
04 April 2018 3,750 2 View
Hello everyone, I have an important question concerning the Huh 7.5 cell line, does Huh 7.5 cells have NTCP receptors or not? you found many papers talking about transfecting the receptor for cell...
08 August 2016 9,637 1 View
Hello everyone, I am really intrested on the mechanisms of virus entry and replication of HBV and HCV in different hepatoma cell lines. I heard about the new established hepatoma cell line,...
05 May 2016 9,900 1 View
Hello everyone, I need to detect core protein (25kD) for HCV and HBx (17kD) from HBV by western blot. I already detected but the band are still not as clear as I want. I used a 12% SDS gel,...
04 April 2016 4,488 4 View
Hello everyone, I have a question concerning the blocking step in Western Blot : Does it make any difference, for the quality of detection, if I change the time of blocking? I used to work with...
04 April 2016 1,412 12 View
Hello everyone, I am trying to detect HBx protein from Hepatitis B Virus through Western Blot. Knowing that the molecluar size of this protein is 17kD can anyone give me an indication about the...
04 April 2016 8,554 2 View
Hello everyone, I am trying to detect hbx and core protein with western blot, is it better to use a 10% or 12% Sds-polyacrylamide gel? Thank you in advance
04 April 2016 2,269 3 View
Hello everyone, can someone tell me the exact differences in the different parameters (florescent dye, number of standards, quantity of the template...) of the real-time PCR for the HBV and HCV...
03 March 2016 2,590 1 View
