HBV replication in Huh-7 cell line?

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Huh 7.5 cell line?

Hello everyone, I have an important question concerning the Huh 7.5 cell line, does Huh 7.5 cells have NTCP receptors or not? you found many papers talking about transfecting the receptor for cell...

07 August 2016 9,677 1 View

Does the hepatoma cell line, HLCZ01, really support HBV and HCV entire life cycle?

Hello everyone, I am really intrested on the mechanisms of virus entry and replication of HBV and HCV in different hepatoma cell lines. I heard about the new established hepatoma cell line,...

04 May 2016 9,933 1 View

Core and HBx proteins western blot detection?

Hello everyone, I need to detect core protein (25kD) for HCV and HBx (17kD) from HBV by western blot. I already detected but the band are still not as clear as I want. I used a 12% SDS gel,...

03 April 2016 4,532 4 View

HBx detection via Western Blot?

Hello everyone, I am trying to detect HBx protein from Hepatitis B Virus through Western Blot. Knowing that the molecluar size of this protein is 17kD can anyone give me an indication about the...

03 April 2016 8,599 2 View

Hbx snd core protein western blot detection?

Hello everyone, I am trying to detect hbx and core protein with western blot, is it better to use a 10% or 12% Sds-polyacrylamide gel? Thank you in advance

03 April 2016 2,311 3 View

Time required for blocking step in Western Blot?

Hello everyone, I have a question concerning the blocking step in Western Blot : Does it make any difference, for the quality of detection, if I change the time of blocking? I used to work with...

03 April 2016 1,461 12 View

HCV and HBV real-time PCR quantification?

Hello everyone, can someone tell me the exact differences in the different parameters (florescent dye, number of standards, quantity of the template...) of the real-time PCR for the HBV and HCV...

02 March 2016 2,641 1 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

How can I apply boundary conditions in an orthotropic steel deck numerical model using ABAQUS software?

I am trying to simulate vehicular loading on an orthotopic steel deck bridge section in ABAQUS software. The red arrow mark in the attached figure indicates the direction in which the vehicle will...

08 August 2024 719 0 View

Unusual intensity drop in some sections of chromatograms in DDA?

Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...

07 August 2024 3,218 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

How to convert a privately loaded document into a public document?

I attempted to make a privately uploaded text public but a window appeared that said an error occurred. There was no explanation provided as to why there was an error or what might be done to...

05 August 2024 8,025 7 View

How to add Cr parameter in autodock?

When I run autogrid4 it says: autogrid4: ERROR: Unknown receptor type: "Cr" -- Add parameters for it to the parameter library first! Look forward to your reply.Thank you so much!

29 July 2024 488 0 View

Hi there, someone has the SeinFit software for windows because I cannot download it?

DOS version.

29 July 2024 6,064 1 View

I need a reliable source or an example supported by excel sheet to understand Fuzzy Vikor?

27 July 2024 5,916 1 View

How to do calculation of Louvain methods calculation for excel sheet and and is there any software available?

The Louvain method – named after the University of Louvain where Blondel et al. developed the algorithm – finds communities by optimizing modularity locally for every node’s neighborhood, then...

23 July 2024 6,659 0 View

Does anyone know a source for theta-replicating Staphylococcus plasmids that are compatible?

I am trying to put 3 separate plasmids into Staphylococcus aureus and I am looking for those that are theta replicating and compatible. I have obtained pSK9065 (from pSK1) from Addgene, but the...

23 July 2024 6,968 0 View