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Questions related from Bruna Rodrigues
We are experienced lately some problems with primary cells. We could see (even in 10x magnification) tiny bright particles around the addhered cells. There is no macroscopic sign of...
12 December 2016 522 12 View
Dear colleagues, I'm needing your help with the protocol of differentiation of mesenchymal stem cells, once I pretty new in this field. I isolated "MSCs" from dental pulp of deciduous teeth using...
06 June 2016 9,441 9 View
I'm tryint to standardize a PCR to mycoplasma detection of dna from cell culture. I'm testing samples positive for mycoplasma and negative as controls. I found a weak band unspecific slightly...
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Could someone send me a good protocol to evaluate markers from mesenchymal stem cells by flow cytometry? if we have to fix the cells before the protocol or not, incubate with fbs or if we have an...
05 May 2016 430 6 View
I have a doubt.My lab has two addresses, and the nitrogen tank to store the cells is at a different address than the cells are grown.Can I use the freezing protocol with Mr. Frosty until the step...
12 December 2015 5,601 3 View
Hi, everyone. I want to isolate mononuclear cells from bone marrow using gradient centrifugation with Ficoll-Paque Premium 1.077g. However, GE's protocol suggests almost 1:1 of ficoll and bone...
10 October 2015 949 5 View