Bruna Rodrigues

6 Questions 23 Answers 0 Followers

Questions related from Bruna Rodrigues

Do we have cell culture contamination?

We are experienced lately some problems with primary cells. We could see (even in 10x magnification) tiny bright particles around the addhered cells. There is no macroscopic sign of...

12 December 2016 525 12 View

Someone with experience in differentiation of MSCs?

Dear colleagues, I'm needing your help with the protocol of differentiation of mesenchymal stem cells, once I pretty new in this field. I isolated "MSCs" from dental pulp of deciduous teeth using...

06 June 2016 9,444 9 View

Could anyone help me with PCR to Mycoplasma detection?

I'm tryint to standardize a PCR to mycoplasma detection of dna from cell culture. I'm testing samples positive for mycoplasma and negative as controls. I found a weak band unspecific slightly...

06 June 2016 4,443 4 View

indication of a good protocol to mesenchymal stem cells by flow cytometry?

Could someone send me a good protocol to evaluate markers from mesenchymal stem cells by flow cytometry? if we have to fix the cells before the protocol or not, incubate with fbs or if we have an...

05 May 2016 436 6 View

Could I transport my cells in the Mr. Frosty in dry ice to another place before placing in liquid nitrogen?

I have a doubt.My lab has two addresses, and the nitrogen tank to store the cells is at a different address than the cells are grown.Can I use the freezing protocol with Mr. Frosty until the step...

12 December 2015 5,606 3 View

Could someone advise me about the best proportion of Ficoll-Paque Premium (1.077g) and bone marrow for isolation of mononuclear cells?

Hi, everyone. I want to isolate mononuclear cells from bone marrow using gradient centrifugation with Ficoll-Paque Premium 1.077g. However, GE's protocol suggests almost 1:1 of ficoll and bone...

10 October 2015 953 5 View