Have you ever used Glu-C endoproteinase to perform in gel digestion?

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How can I calculate the diffusion of water in a sphere (radial diffusion)?

I am measuring the moisture absorption of PET granules by measuring the mass over time. Is it possible to approximate numerically the integrated Fick's law (mass vs time) for the case of the sphere?

22 December 2020 6,359 3 View

Is it possible to fix the intercept in a linear model (lm() in R)?

Dear all, I believe this should be a simple question but after trying and reading I still have no solutions. Basically what I want to do is to run a linear model in R fixing the intercept to a...

26 November 2020 6,897 6 View

Which non-parametric equivalent to the two way ANOVA can be used?

Dear colleagues, I am looking for a non-parametric alternative to the two-way ANOVA as my data are not normally distributed (variances are homogeneous). Friedman test is not a good option as my...

28 September 2020 9,857 11 View

Is it possible to calculate diffusion coefficient through a porous thin film using mass and time?

Hello, I have some porous coating on a hygroscopic substrate. I am measuring the mass change over time of samples left in water. The samples were previously dried. Is it possible to derive from...

09 September 2020 8,818 3 View

Molecular SET-based circuit simulation tools?

Hi! Are there any simulation tool for digital circuits based on Single Electron Transistor (SET) where the dot is single molecule? Is SIMON simulator technology dependent? Thank you

29 May 2020 1,493 3 View

How to properly set k-points in atomistic transport calculations?

Hi I'm interested in analyzing transport in nanodevices. I don't clearly understand how to properly set the number of k-points sampling in transmission spectrum computations. Is it possible, given...

09 March 2020 1,327 4 View

How can I dry an aqueous suspension of polymer nanoparticles?

Can I try to use vacuum oven to obtain a powder that I can then redisperse? Thanks

18 February 2020 3,802 2 View

Does anybody have experience with Igor Pro on macOS 10.15 (Catalina)?

WaveMetrics recommends Igor Pro users to avoid upgrading to Catalina. However, this is only due to the fact that third party XOPs may not run. I therefore wonder if anybody have tried to use Igor...

12 November 2019 6,237 1 View

Why can't I get bands after SDS PAGE?

Hello everyone. I run a SDS PAGE on wheat anthers for my fist time, and I have got this outcome (see annex). In the first line (left to right), I loaded 10ul of ladder ( Spectra Multicolor Broad...

08 October 2019 7,439 8 View

Colour of PVP in water?

I have prepared a solution of PVP (Mw=10k) in distilled water. After sonication for 30 min and 1 day of stirring, the colour of the solution is brownish. Is It correct? Thanks.

05 December 2018 6,804 1 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

What are some important techniques for protein induction and SDS-PAGE analysis?

Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...

03 March 2021 8,237 3 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View