Have you ever used Glu-C endoproteinase to perform in gel digestion?

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Non-parametric ANCOVA post hoc test?

I am doing a non-parametric ANCOVA (my residuals are not normally distributed and transformation did not help), I have been using the sm.ancova function from the sm package. Now I want to do a...

07 April 2024 8,974 5 View

I would like to have the following paper?

Investigation on ejection dynamics of air launched missile

06 April 2024 7,434 1 View

Can i have copy of the paper Investigation on ejection dynamics of air launched missile?

I am working on a similar projects and want to have copy of your paper

06 April 2024 2,764 1 View

I would like to have the following paper?

Investigation on ejection dynamics of air launched missile and the following as of the failure

06 April 2024 3,731 1 View

Both negative and positive clone in my colony PCR?

Hello everyone, I've been trying to clone a bacterial protein from S. aureus into E. coli with pQE30 vector. After ligation and transformation (with Xl1Blue), I screened my colonies (more than...

27 March 2024 1,602 3 View

How to reach the target pH in sterile broth after pre-reduction in the anaerobic cabinet?

Hello, I need your support and suggestions. When I autoclave broth media and put them in my anaerobic cabinet to pre-reduce, the pH will drop of 0.5-1.0 (depending on the medium composition and...

22 February 2024 2,810 1 View

Could you please share any recommendations for works such as books, articles that delve into "work as a masculinity contest" ?

Hi! I am currently working on a research project focused on exploring the dynamics of masculinity within the context of the workplace, specifically delving into the concept of "work as a...

23 January 2024 2,261 3 View

I put in freezer antibodies that must be stored in fridge. Someone can help me?

I bought from Epigentek the EpiQuik DNMT Activity/Inhibition ELISA Easy Kit P-3139-48 and the CA capture antibody must be stored at 4°C but I put in at - 20 °C. I bought from Epigentek the HDAC...

19 December 2023 4,682 2 View

Why SSRs show low recombination fraction in linkage mapping based on SNPs?

Hi, I am building grapevine high-density genetic maps based on SNPs. I have one F1 population and I am using the pseudo-test cross method. I am trying to insert SSRs but, despite the fact that...

14 December 2023 6,245 0 View

Which is the minimum number of images to be taken in immunofluorescence assays?

I need to quantify images from immunofluorescence assays. Which is the minimum number of images to be taken when I take into account the reactivity area of positive cells to a specific marker? If...

30 July 2023 3,417 3 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

How to preform densitometry on SDS-page bands?

I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...

05 August 2024 9,805 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Could someone explain the basics of working with enzymes?

I'm interested in learning about enzyme usage. Could someone explain the basics of working with enzymes? I understand they're different from standard chemicals, but I'm uncertain about the...

01 August 2024 5,460 5 View