What is the institute/organization that provides training for hydroponics development ?

I also want to know the organization working on development of nutrient solution for hydroponics and the organization providing training on it.

02 June 2020 8,901 4 View

Is there a way to correlate one-dimensional netcdf data (like a time-series data) with the spatial gridded dataset (which is multi-dimensional)?

For eg. I have a netcdf file which is in the form of a time-series and the other data is a gridded .nc file for geopotential height. I want to correlate both of these datasets. Please guide me...

27 May 2020 8,133 3 View

T-cell from frozen spleen?

Hello, I have some frozen spleen from young and old mice and wanted to isolate T-cells from these spleens. These spleens were snap-frozen and stored in -80. They were never thawed. So, I was...

12 May 2020 4,810 3 View

What kind of properties are needed in ANSYS or other FEA packages to conduct the stress/strain analysis of 2D materials under tension, and bending?

I want to simulate the effect of stresses on a graphene monolayer and observe the deformations appearing in the material. The problem is the understanding of the element size and how to define a...

02 August 2019 9,005 4 View

IP with HSF1 antibody in liver tissue??

Hi, I am trying to perform IP with HSF1 antibody from liver tissue but no success so far. I prepared my tissue lysate in RIPA buffer and I am using 1mg protein for IP and bead used is protein A/G...

04 February 2019 9,691 7 View

I am working on Brain tumor segmentation/detection using ANN,watershed and morphological operations but i lack images with grand truth any help?

I need small Training and test data with valid grand truth for my MS.c project regardless synthetic or real, if anyone can point me in right direction i would really appreciate it. "BRATS" isn't...

24 August 2016 2,757 4 View

What's the best method/alternative to wirebond to flexible mica substrates?

I have bondpands patterned on flexible mica substrates (cleaved thin disks of muscovite mica, ~100um thick). The pads are usually e-beam evaporated Ti/Au or Ti/Pd metal stacks of thickness 5/100...

06 July 2016 9,883 3 View

Trying to detect fluorescent labeled S. aureus in media containing lysed erythrocytes and plasma. What parameters should one use?

S. aureus cells are labeled with mCherry or GFP, cannot use side scatter analysis because of lysed erythrocytes. Need to detect specific cell counts based on flourescence events for number of cell...

11 May 2016 3,322 3 View

How to prevent cell clumping in matrigel invasion assay?

I am working with a cell line that migrates very well in transwell migration assays, but when I try to do an invasion assay, the cells clump together within 2-3 hours after plating. I plate them...

05 May 2016 6,261 2 View

Which part of the body/tissue is best for DNA isolation in relation to DNA Barcoding of marine molluscs?

05 September 2015 7,739 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View