Is it possible to obtain SEM images of the surface of the electrically insulating thin film, which is 200 microns thick and has a pale yellow color, without detaching it from the 4 mm thick glass substrate?
To avoid the charging of the surface, it is necessary to produce a conductive surface by evaporation or sputtering (see @Insic). My recommendation would be some nm Au. The thickness of this metallic layer must be lower than the reach (penetration depth) of secondary electrons (SEM-mode). Look for penetration depth of electrons with energies < 50 eV. In the case of thicker layers you observe only backscattered electrons (BSE-mode).
Don't forget to ground the surface (Connect the surface layer with zero Potential).
For better SEM images ITO coated glass is preffered with the thickness of glass substrate about 3mm where as the coating material should ranges from 180-220 microns. Later on Use silver Paste to make electrical connections.