Kalkal Anil Trivedi

4 Questions 12 Answers 0 Followers

Questions related from Kalkal Anil Trivedi

How to prevent cell clumping in matrigel invasion assay?

I am working with a cell line that migrates very well in transwell migration assays, but when I try to do an invasion assay, the cells clump together within 2-3 hours after plating. I plate them...

06 May 2016 6,339 2 View

How can cellular ATP be increased in cell culture conditions?

I need to study the effect of increase in cellular ATP on the expression of a protein. How can this be achieved in in vitro conditions? I work with cancer cell lines like HeLa.

29 January 2014 4,544 5 View

Have there been any reports of interaction of caffeine and adenosine other than on adenosine receptors?

Caffeine and Adenosine interactions other than at Adenosine receptors with theparticular effect on intracellular signalling.

16 October 2013 8,297 1 View

Dark bands on edges of Western Blot and faint development of bands in the center of WB, even in the loading control?

I am trying to replicate an experiment previously conducted, and observed that irrespective of the samples loaded, on developing the WB by chemiluminescent substrate, the bands on both edges of...

17 September 2013 5,747 42 View