Kalkal Anil Trivedi

4 Questions 11 Answers 0 Followers

Questions related from Kalkal Anil Trivedi

How to prevent cell clumping in matrigel invasion assay?

I am working with a cell line that migrates very well in transwell migration assays, but when I try to do an invasion assay, the cells clump together within 2-3 hours after plating. I plate them...

06 May 2016 6,319 2 View

How can cellular ATP be increased in cell culture conditions?

I need to study the effect of increase in cellular ATP on the expression of a protein. How can this be achieved in in vitro conditions? I work with cancer cell lines like HeLa.

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Have there been any reports of interaction of caffeine and adenosine other than on adenosine receptors?

Caffeine and Adenosine interactions other than at Adenosine receptors with theparticular effect on intracellular signalling.

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Dark bands on edges of Western Blot and faint development of bands in the center of WB, even in the loading control?

I am trying to replicate an experiment previously conducted, and observed that irrespective of the samples loaded, on developing the WB by chemiluminescent substrate, the bands on both edges of...

17 September 2013 5,739 42 View