Dear Researchers,
I am using Amplex® Red Hydrogen Peroxide/Peroxidase Assay Kit (Thermo Fischer) for H2O2 quantification from cell. There is an option for Qubit but, there is no clear protocol for the quantification of H2O2 from cell using Qubit. I am confused. How should I use it for cell using Qubit? Thanks in advance.
Amplex red is unstable in the presence of instrumental excitation light and can generate the fluorescent resorufin in the absence of H2O2 and HRP. The generation of the superoxide radical and H2O2 are artefacts of photooxidation (Zhao et al. 2012), Therefore, the method of Aebi (Aebi 1984), and Beers (Beers and Sizer 1952) should be used. If necessary run both in parallel to confirm this
Amplex red is unstable in the presence of instrumental excitation light and can generate the fluorescent resorufin in the absence of H2O2 and HRP. The generation of the superoxide radical and H2O2 are artefacts of photooxidation (Zhao et al. 2012), Therefore, the method of Aebi (Aebi 1984), and Beers (Beers and Sizer 1952) should be used. If necessary run both in parallel to confirm this
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