How many cells I need to make protein sample from primary hepatocyte culture for WB analysis?

I need to check protein expression in primary hepatocytes after some treatment through WB analysis. I am facing problem regarding amount of protein in cell homogenate. The protein concentration...

07 August 2019 6,042 9 View

How to raise primary antibody against my protein of interest?

I want to raise primary antibody against my protein of interest in my lab. I need help to make it. I have full length mRNA sequence of my gene. Can you provide me any protocol to raise primary...

08 September 2018 8,840 6 View

How to check tissue specific distribution of mRNA using Real-time PCR?

I just want to check the expression of a particular mRNA throughout the different tissues (Liver, Kidney, Brain, Muscle, Gills, Intestine, Ovary, Skin, Spleen etc.) in a control/untreated fish. If...

01 February 2018 5,235 7 View

Why only BSA and Skimmed milk proteins are used for blocking in Western blotting?

Why antibodies (primary and secondary) do not cross react with these protein? What chemistry is there to choose these proteins as blocking reagent?

31 December 2017 3,481 7 View

Do the dilution factor of antibody (primary and secondary) should be same for endogenous control and target protein?

The same dilution factor of antibody doesn't fit for the endogenous and target protein. I am working on fish model and the primary antibody which I am using for catalase (CAT) protein needs...

09 October 2017 3,457 3 View

No title

10 November 2016 6,867 2 View

What is the difference in the different parts of fish kidney in terms of functional anatomy ?

07 August 2016 5,335 0 View

Is it recommended to use 0.5X SYBR Green Master Mix?

07 August 2016 5,234 8 View

What is the optimum concentration of Nitric Oxide (NO) in brain tissue of Catfish (Clarias batrachus)?

I have quantified NO in different tissues of catfish (Clarias batrachus) using Griess reagent. I am getting very high amount of NO (>300 nmoles/ gm wet wt). I have followed the steps...

07 August 2016 4,631 1 View

What is the significance of molar extinction coefficient in enzyme kinetics assay?

What does it actually imply?

31 December 2015 6,279 3 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Can chitosan alone can act as a reducing agent for Cu(0) nanoparticle sythesis?

[Synthesizing Cu nanoparticles without using chemical reductants]

01 March 2021 8,359 1 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View