How many cells I need to make protein sample from primary hepatocyte culture for WB analysis?

I need to check protein expression in primary hepatocytes after some treatment through WB analysis. I am facing problem regarding amount of protein in cell homogenate. The protein concentration...

07 August 2019 6,042 9 View

How to synthesis PbO nanoparticles by chemical process?

I want to make PbO nanoparticle through chemical process. Can anyone provide me the protocol. Thanks in advance

08 September 2018 6,110 2 View

How to raise primary antibody against my protein of interest?

I want to raise primary antibody against my protein of interest in my lab. I need help to make it. I have full length mRNA sequence of my gene. Can you provide me any protocol to raise primary...

08 September 2018 8,840 6 View

How to check tissue specific distribution of mRNA using Real-time PCR?

I just want to check the expression of a particular mRNA throughout the different tissues (Liver, Kidney, Brain, Muscle, Gills, Intestine, Ovary, Skin, Spleen etc.) in a control/untreated fish. If...

01 February 2018 5,235 7 View

Why only BSA and Skimmed milk proteins are used for blocking in Western blotting?

Why antibodies (primary and secondary) do not cross react with these protein? What chemistry is there to choose these proteins as blocking reagent?

31 December 2017 3,481 7 View

Do the dilution factor of antibody (primary and secondary) should be same for endogenous control and target protein?

The same dilution factor of antibody doesn't fit for the endogenous and target protein. I am working on fish model and the primary antibody which I am using for catalase (CAT) protein needs...

09 October 2017 3,457 3 View

No title

10 November 2016 6,867 2 View

Is it recommended to use 0.5X SYBR Green Master Mix?

07 August 2016 5,234 8 View

What is the optimum concentration of Nitric Oxide (NO) in brain tissue of Catfish (Clarias batrachus)?

I have quantified NO in different tissues of catfish (Clarias batrachus) using Griess reagent. I am getting very high amount of NO (>300 nmoles/ gm wet wt). I have followed the steps...

07 August 2016 4,631 1 View

What is the significance of molar extinction coefficient in enzyme kinetics assay?

What does it actually imply?

31 December 2015 6,279 3 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

Is there a deep reason for using the squared modulus of the wave function as the probability density of the existence of the particle?

I think we were all surprised at the first time we got to know quantum mechanics that the squared modulus of the wave function is the probability density of the existence of the particle? The role...

28 February 2021 6,282 9 View