What is the optimum concentration of Nitric Oxide (NO) in brain tissue of Catfish (Clarias batrachus)?

08 August 2016 1 5K Report

I have quantified NO in different tissues of catfish (Clarias batrachus) using Griess reagent. I am getting very high amount of NO (>300 nmoles/ gm wet wt). I have followed the steps below-

Homogenized 100 mg fresh tissues to make 10% homogenate.
Centrifuged at 10000 g for 10 mins.
Taken the supernatant and added same volume of 10% PCA.
Centrifuged at 10000 g for 10 mins.
Taken the supernatant for quantification.

What is the normal quantity of NO present in brain. Is it very high value (seems to me) of NO in brain?

Thanks in advance.

S.I.R. Okoduwa

So many conditions must be consider in answering your question. You need to give more details as per the state of the animal involved

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