have a protocol for RNA extraction from whole blood usig TRIazol reagent and RBCs lysis buffer?? aomplished by RBCs lysis buffer recipe
You can try this..
10 mM Tric-Cl (pH 8.0), 0.1 M EDTA (pH 8.0), 0.5% (w/v) SDS, 20 microgram/ml DNase-free RNase.
Please I want to know if there is primer dimer in this curve??
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is there any web site at which i can check my primers for spanning exon exon junctions?
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is there any experimental procedures to confirm the presence of cDNA after reverse transcription before pcr??
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I wonder if I can reuse TRIazol reagent after extracting RNA using a specific volume of it after removing of the chloroform layer
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see above
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