Please I want to know if there is primer dimer in this curve??

Please I want to know if there is primer dimer in this curve??

06 July 2016 7,070 6 View

How could i perform relative quantification for real time pcr?

Does any one knows how to analyze data from real time pcr using execl? I mean relative quantrelative quantification?

05 June 2016 7,480 1 View

Is there any web site at which i can check my primers for spanning exon exon junctions?

is there any web site at which i can check my primers for spanning exon exon junctions?

01 February 2016 1,651 1 View

Are there any experimental procedures to confirm the presence of cDNA after reverse transcription before pcr?

is there any experimental procedures to confirm the presence of cDNA after reverse transcription before pcr??

31 December 2015 3,192 13 View

Can I reuse TRIazol reagent for extracting RNA from another sample?

11 December 2014 4,791 0 View

Have a protocol for RNA extraction from whole blood usig TRIazol reagent and RBCs lysis buffer?? aomplished by RBCs lysis buffer recipe

have a protocol for RNA extraction from whole blood usig TRIazol reagent and RBCs lysis buffer?? aomplished by RBCs lysis buffer recipe

08 September 2014 4,537 1 View

Plz give me a guidance about ethanol preciptation??

Hi, Plz anyone could give me his or her guidance about protein preciptation using ice cold ethanol. My question is Does ice cold ethanol preciptation of proteins in RIPA lysis buffer affect...

31 December 2011 6,706 4 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View