Please I want to know if there is primer dimer in this curve??

Please I want to know if there is primer dimer in this curve??

06 July 2016 7,070 6 View

How could i perform relative quantification for real time pcr?

Does any one knows how to analyze data from real time pcr using execl? I mean relative quantrelative quantification?

05 June 2016 7,480 1 View

Are there any experimental procedures to confirm the presence of cDNA after reverse transcription before pcr?

is there any experimental procedures to confirm the presence of cDNA after reverse transcription before pcr??

31 December 2015 3,192 13 View

Can I reuse TRIazol reagent for extracting RNA from another sample?

11 December 2014 4,791 0 View

Have a protocol for RNA extraction from whole blood usig TRIazol reagent and RBCs lysis buffer?? aomplished by RBCs lysis buffer recipe

have a protocol for RNA extraction from whole blood usig TRIazol reagent and RBCs lysis buffer?? aomplished by RBCs lysis buffer recipe

08 September 2014 4,537 1 View

What is the best protocol for RNA extraction from whole blood using TRIZol reagent?

see above

02 March 2014 3,305 4 View

Plz give me a guidance about ethanol preciptation??

Hi, Plz anyone could give me his or her guidance about protein preciptation using ice cold ethanol. My question is Does ice cold ethanol preciptation of proteins in RIPA lysis buffer affect...

31 December 2011 6,706 4 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View