I wonder if I can reuse TRIazol reagent after extracting RNA using a specific volume of it after removing of the chloroform layer
Please I want to know if there is primer dimer in this curve??
06 July 2016 7,114 6 View
Does any one knows how to analyze data from real time pcr using execl? I mean relative quantrelative quantification?
05 June 2016 7,525 1 View
is there any web site at which i can check my primers for spanning exon exon junctions?
01 February 2016 1,694 1 View
is there any experimental procedures to confirm the presence of cDNA after reverse transcription before pcr??
31 December 2015 3,279 13 View
have a protocol for RNA extraction from whole blood usig TRIazol reagent and RBCs lysis buffer?? aomplished by RBCs lysis buffer recipe
08 September 2014 4,576 1 View
see above
02 March 2014 3,383 4 View
Hi, Plz anyone could give me his or her guidance about protein preciptation using ice cold ethanol. My question is Does ice cold ethanol preciptation of proteins in RIPA lysis buffer affect...
31 December 2011 6,748 4 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?
03 August 2024 7,829 0 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View