I wonder if I can reuse TRIazol reagent after extracting RNA using a specific volume of it after removing of the chloroform layer
Please I want to know if there is primer dimer in this curve??
06 July 2016 7,070 6 View
Does any one knows how to analyze data from real time pcr using execl? I mean relative quantrelative quantification?
05 June 2016 7,480 1 View
is there any web site at which i can check my primers for spanning exon exon junctions?
01 February 2016 1,651 1 View
is there any experimental procedures to confirm the presence of cDNA after reverse transcription before pcr??
31 December 2015 3,192 13 View
have a protocol for RNA extraction from whole blood usig TRIazol reagent and RBCs lysis buffer?? aomplished by RBCs lysis buffer recipe
08 September 2014 4,537 1 View
see above
02 March 2014 3,305 4 View
Hi, Plz anyone could give me his or her guidance about protein preciptation using ice cold ethanol. My question is Does ice cold ethanol preciptation of proteins in RIPA lysis buffer affect...
31 December 2011 6,706 4 View
dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...
03 March 2021 6,994 5 View
Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue
02 March 2021 5,433 5 View
I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...
28 February 2021 7,370 3 View
I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...
28 February 2021 4,949 3 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...
27 February 2021 9,233 3 View
Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...
25 February 2021 1,660 3 View
I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?
25 February 2021 9,761 1 View
Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...
25 February 2021 3,612 3 View
Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...
24 February 2021 5,749 3 View