Has anyone used a premade hybridization buffer (from Roche for example) for zebrafish in situ hybridizations?

More Curtis R French's questions See All

Regarding cement based pore structure. How do you calculate pore volume using electrical conductivity/resistivity measurements?

I am trying to calculate pore size distribution of concrete using electrical resistivity measurements however I am unable to calculate the volume of the pore structure. Can anyone advise on how I...

19 January 2020 8,683 2 View

Does anybody have any resources to understand how polymerization kinetics of random copolymers work? Specifically hydrogels if possible?

I am performing rate of reaction studies on hydrogels with 2 monomers and crosslinker. We can look at absorbance of a single wavelength of light over the course of the reaction through UV-Vis to...

29 October 2019 4,443 2 View

What disease pathologies are you aware of that would benefit from elemental analysis?

Hello, I am currently researching a spectral computed tomography system for medical applications. The system excels in delineating the elemental composition of materials, providing a 3D...

06 March 2019 3,465 3 View

Making antisense probe from topo?

I need to make an antisense probe from a cloned sequence in pcrTOPO4. When I sequenced using the m13R primer, the sequence through a BLAST search come back as Plus/Minus. Just wondering how i...

01 March 2017 3,003 3 View

Will anti DIG-AP (roche) work after one freeze thaw cycle?

My anti DIG antibody-AP (Fab fragments, ROCHE, ) were were put in our freezer upon delivery. The product information states to store at 4 degress, and specifically says not to freeze. Anyone know...

27 September 2016 278 2 View

Lipid extraction for samples stored in ethanol?

Hello, I'm hoping to extract lipids from some shark muscle samples for some fatty acid analyses. All the literature I can find is based on frozen samples, but mine are stored in ethanol - will...

02 March 2016 9,921 4 View

Any recommendations on which macrophage (P388D1 vs RAW 264.7) to use for studying bacterial killing via the oxidative burst?

I have a mutant bacteria that is more tolerant to H2O2 and I would like to test if it is more persistent in the macrophage model? Will either of these cell lines phagocytose and degrade bacteria,...

23 March 2015 9,088 4 View

Can anyone suggest reasons for the appearance of this non-specific band in PCR?

I am working on amplifying the nirK gene from whole community genomic DNA. However I am getting a second band just below my target (500bp) band. The PCR program I am using is below: 94C-5min |...

03 February 2014 7,182 71 View

Great project! How did you go about warming the students up to the rich picturing process, to get them involved?

How did you go about warming the students up to the rich picturing process, to get them involved? I feel that I have never quite cracked the challenge and disbelief from participants in RP...

01 January 1970 1,738 3 View

The use of artificial intelligence in photogrammety for cultural heritage

I'm writing a dissertation on the use of artificial intelligence to enhance the cultural heritage applications of photogrammetry. I was inspired by Yves Ubelman's work in Palmyra (as featured on...

01 January 1970 9,536 5 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Bryan Douglas Crawford

I've always used home-made hybjuice but I can't imagine why commercial products wouldn't work. I would suggest you do at least one 50/50 incubation with whatever buffers you're using before and after the hybridization steps so you're not slamming your tissues from one solvent condition to another. Good luck!