Dear colleagues- we routinely record local field potentials in freely moving mice from hippocampal CA1 and prefrontal cortex with ~ 125um depth electrodes, but would like to record multi neuronal single unit activity (and LFP's) in lightly restrained or freely moving animals. We would like to use silicon based shank electrodes with 16 to 32 recording sites, with one and sometimes two shanks- we have Intan recording systems. We have found some possible commercial solutions, but they are quite expensive and seem to be single use only. I was wondering if anyone had come across some more "reasonably priced" silicon or similar arrays? I am aware of tetrode methods and the more advanced CMOS based electrodes, but think the silicon array would be best suited to our current studies.
Usually we design the electrodes and record the oscillations from 4 or 5 different regions of the brain. During the process of fixing them, we use a hardening material such as cement with thinner fluid, which dries quickly and becomes very solid and is suitable for maintaining the electrodes during recording with movement
These articles may provide you some useful information
1. Ahmadvand, Tala, Sara Mirsadeghi, Faezeh Shanehsazzadeh, Sahar Kiani, and Mehdi Fardmanesh. 2020. "A Novel Low-Cost Method for Fabrication of 2D Multi-Electrode Array (MEA) to Evaluate Functionality of Neuronal Cells" Proceedings 60, no. 1: 51. https://doi.org/10.3390/IECB2020-07087
2. Pelkonen, Anssi, Cristiana Pistono, Pamela Klecki, Mireia Gómez-Budia, Antonios Dougalis, Henna Konttinen, Iveta Stanová et al. "Functional Characterization of Human Pluripotent Stem Cell-Derived Models of the Brain with Microelectrode Arrays." Cells 11, no. 1 (2022): 106.
3.Pérez-Prieto, Norberto, and Manuel Delgado-Restituto. "Recording strategies for high channel count, densely spaced microelectrode arrays." Frontiers in Neuroscience (2021): 869.
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