Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Has anyone used a premade hybridization buffer (from Roche for example) for zebrafish in situ hybridizations?

Our in situs (alkaline phosphatase based) have stopped working. Probes are fine, but or homemade hybridization buffer seems to be the issue. Using the same supplier of deionized formaminde as...

03 February 2021 8,540 2 View

Does anybody have any resources to understand how polymerization kinetics of random copolymers work? Specifically hydrogels if possible?

I am performing rate of reaction studies on hydrogels with 2 monomers and crosslinker. We can look at absorbance of a single wavelength of light over the course of the reaction through UV-Vis to...

29 October 2019 4,443 2 View

Experiment conditions for ELISA using the purified protein and synthetic peptide?

Hi. I'm planning to perform ELISA to find amino acid residues which directly bind to the protein X (protein of interest). You may see the design of the experiment in the picture that I posted....

07 August 2019 2,536 3 View

What disease pathologies are you aware of that would benefit from elemental analysis?

Hello, I am currently researching a spectral computed tomography system for medical applications. The system excels in delineating the elemental composition of materials, providing a 3D...

06 March 2019 3,465 3 View

Making antisense probe from topo?

I need to make an antisense probe from a cloned sequence in pcrTOPO4. When I sequenced using the m13R primer, the sequence through a BLAST search come back as Plus/Minus. Just wondering how i...

01 March 2017 3,003 3 View

Will anti DIG-AP (roche) work after one freeze thaw cycle?

My anti DIG antibody-AP (Fab fragments, ROCHE, ) were were put in our freezer upon delivery. The product information states to store at 4 degress, and specifically says not to freeze. Anyone know...

27 September 2016 278 2 View

Why does the commercial zeolite form agglomerate?

From the various journal, and my prepared samples, I have seen various zeolite particles with SEM. Lab made zeolite was not agglomerated after hydrothermal process and calcination but commercial...

29 May 2016 5,280 1 View

Any recommendations on which macrophage (P388D1 vs RAW 264.7) to use for studying bacterial killing via the oxidative burst?

I have a mutant bacteria that is more tolerant to H2O2 and I would like to test if it is more persistent in the macrophage model? Will either of these cell lines phagocytose and degrade bacteria,...

23 March 2015 9,088 4 View

Can anyone suggest reasons for the appearance of this non-specific band in PCR?

I am working on amplifying the nirK gene from whole community genomic DNA. However I am getting a second band just below my target (500bp) band. The PCR program I am using is below: 94C-5min |...

03 February 2014 7,182 71 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

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03 March 2021 5,360 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View