Curtis R French

4 Questions 2 Answers 0 Followers

Questions related from Curtis R French

Has anyone used a premade hybridization buffer (from Roche for example) for zebrafish in situ hybridizations?

Our in situs (alkaline phosphatase based) have stopped working. Probes are fine, but or homemade hybridization buffer seems to be the issue. Using the same supplier of deionized formaminde as...

04 February 2021 8,757 2 View

Making antisense probe from topo?

I need to make an antisense probe from a cloned sequence in pcrTOPO4. When I sequenced using the m13R primer, the sequence through a BLAST search come back as Plus/Minus. Just wondering how i...

02 March 2017 3,159 3 View

Will anti DIG-AP (roche) work after one freeze thaw cycle?

My anti DIG antibody-AP (Fab fragments, ROCHE, ) were were put in our freezer upon delivery. The product information states to store at 4 degress, and specifically says not to freeze. Anyone know...

28 September 2016 435 2 View

Has anyone used the TriTech analog microinjection system for zebrafish embryos? Any reviews?

This is a low cost system for attachment to a compressed air tank.

03 March 2016 2,194 1 View