I am trying to reprogram a mouse liver cancer cell line to iPSC. About 2 weeks after transfection I started seeing mouse ES-like colonies. I dissociated and replated them on new feeders hoping to get more colonies, but what I saw were not colonies, rather cells with morphology similar to the original cancer cells. May I ask what those colonies might be?
EMT and you can study some modifications on the molecular pathways .
It can be done with some cancer types, for example MLL-AF9 leukemia, which is largely driven by epigenetic aberration.
http://www.nature.com/leu/journal/v28/n5/full/leu2013304a.html
However, some driver mutations/rearrangements may prevent the iPSC from being as malleable as other non-cancerous cells in terms of reprogramming potential.
I think it's not a real colonies. The cells are not fully reprogrammed. This is not a successful experiment
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